Cell Death Techniques - Couverture souple

 
9781621820055: Cell Death Techniques
Couverture souple
ISBN 10 : 162182005X ISBN 13 : 9781621820055
Editeur : Cold Spring Harbor Laboratory Press,U.S., 2015

Synopsis

Regulated cell death, which is involved in nearly every aspect of animal development and physiology, can be challenging to study in the laboratory. There are multiple types of cell death, several interconnected signaling pathways can be activated, and the postmortem consequences may vary. Therefore, a combination of specialized techniques is often required to characterize and confirm cell death in the system of interest.
This laboratory manual provides a comprehensive suite of step-by-step protocols for inducing, detecting, visualizing, characterizing, and quantifying cell death in a variety of systems. The contributors describe strategies for identifying and evaluating the biochemical and morphological changes that occur during apoptosis, autophagy, necrosis, and other forms of cell death, as well as for pinpointing the genes and proteins that may be involved. The protocols employ flow cytometry, imaging and microscopy, RNAi, western blotting, enzymatic activity measurements, and other tools to assess phenomena such as DNA fragmentation, lysosomal membrane permeabilization, autophagic flux, apoptosome or ripoptosome formation, caspase activation, and cytochrome c release. Techniques are described for both in vitro systems and in vivo models, including mice, C. elegans, and Drosophila.
The authors also provide guidance on interpreting and presenting the results of cell death experiments, as well as advice on complementary procedures that may be required to confirm the results of a given experiment. This indispensable laboratory resource will enable all cell and developmental biologists, from the graduate level upward, to confidently carry out and comprehend a wide array of cell viability assays.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Regulated cell death, which is involved in nearly every aspect of animal development and physiology, can be challenging to study in the laboratory. There are multiple types of cell death, several interconnected signaling pathways can be activated, and the postmortem consequences may vary. Therefore, a combination of specialized techniques is often required to characterize and confirm cell death in the system of interest.
This laboratory manual provides a comprehensive suite of step-by-step protocols for inducing, detecting, visualizing, characterizing, and quantifying cell death in a variety of systems. The contributors describe strategies for identifying and evaluating the biochemical and morphological changes that occur during apoptosis, autophagy, necrosis, and other forms of cell death, as well as for pinpointing the genes and proteins that may be involved. The protocols employ flow cytometry, imaging and microscopy, RNAi, western blotting, enzymatic activity measurements, and other tools to assess phenomena such as DNA fragmentation, lysosomal membrane permeabilization, autophagic flux, apoptosome or ripoptosome formation, caspase activation, and cytochrome c release. Techniques are described for both in vitro systems and in vivo models, including mice, C. elegans, and Drosophila.
The authors also provide guidance on interpreting and presenting the results of cell death experiments, as well as advice on complementary procedures that may be required to confirm the results of a given experiment. This indispensable laboratory resource will enable all cell and developmental biologists, from the graduate level upward, to confidently carry out and comprehend a wide array of cell viability assays.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Cold Spring Harbor Laboratory Press,U.S.
Date d'édition
2015
Langue
anglais
ISBN 10 
162182005X
ISBN 13 
9781621820055
Reliure
Broché
Nombre de pages
514
Éditeur
Johnstone Ricky, Silke John
Coordonnées du fabricant
non disponible
Personne responsable
non disponible

Autres éditions populaires du même titre

9781621820123: Cell Death Techniques: A Laboratory Manual

Edition présentée

ISBN 10 :  1621820122 ISBN 13 :  9781621820123
Editeur : Cold Spring Harbor Laboratory Pr..., 2015
Couverture rigide