Synopsis
1. Conventional Murine Gene Targeting
Albert G. Zimmermann and Yue Sun
2. Production and Characterization of Humanized Rag2-/-γc-/- Mice
Freddy M. Sanchez, German I. Cuadra, Stanton J. Nielsen, Anne Tanner, and Bradford K. Berges
3. Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages
Beckley K. Davis
4. Collecting Resident or Thioglycollate Elicited Peritoneal Macrophages
Monika Schneider
5. Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils
Linda Vong, Philip M Sherman, and Michael Glogauer
6. Assessment of Oxidative Metabolism
Emilie Imbeault and Denis Gris
7. Generation and Culture of Mouse Embryonic Fibroblasts
Yu Lei
8. Primary Ear Fibroblast Derivation from Mice
Chris B. Moore and Irving C. Allen
9. Bone Marrow Derived Dendritic Cells
Kelly Roney
10. Measuring T Cell Function in Innate Immune Models
Brianne R. Barker
11. Bioassay for the Measurement of Type-I Interferon Activity
Douglas G. Widman
12. Safe and Effective Mouse Foot-Pad Inoculation
Kristin M. Long and Mark Heise
13. Delayed Type Hypersensitivity Models in Mice
Irving C. Allen
14. Mouse Model of Staphylococcus aureus Skin Infection
Natalia Malachowa, Scott D. Kobayashi, Kevin R. Braughton, and Frank R. DeLeo
15. Sepsis Induced by Cecal Ligation and Puncture
Haitao Wen
16. Systemic Infection of Mice with Listeria monocytogenes to Characterize Host Immune Responses
Nancy Wang, Richard A. Strugnell, Odilia L. Wijburg, and Thomas C. Brodnicki
17. Mouse Model of Invasive Fungal Infection
Donna M. MacCallum
18. Endotoxin-Induced Uveitis in Rodents
Umesh C.S. Yadav and Kota V. Ramana
19. Bacteria-Mediated Acute Lung Inflammation
Irving C. Allen
20. Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome
Catherine J. Sanders, Brian Johnson, Charles W. Frevert, and Paul G. Thomas
21. Dextran Sodium Sulfate-Induced Murine Inflammatory Colitis Model
Monika Schneider
22. Bacterial-Mediated Gastrointestinal Inflammation
Joshua Uronis and Xiaolun Sun
23. Plasmodium berghei ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria
Marcela Montes de Oca and Ashraful Haque
24. Characterization of HIV-1 Infection in the Humanized Rag2-/-γc-/- Mouse Model
Freddy M. Sanchez and Bradford K. Berges
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Mouse Models of Innate Immunity : Methods and Protocols
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Hardcover. Etat : new. Hardcover. The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response. The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; Shipping may be from multiple locations in the US or from the UK, depending on stock availability. N° de réf. du vendeur 9781627034807
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Mouse Models of Innate Immunity
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Buch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response. 240 pp. Englisch. N° de réf. du vendeur 9781627034807
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Gebunden. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes detailed contributions from a diverse group of authorsFeatures both ex vivo and in vivo protocols that are lab-readyContains expert tips and key implementation advice to ensure successful resultsThe innate immune system . N° de réf. du vendeur 4267636
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Etat : New. Editor(s): Allen, Irving C. Series: Methods in Molecular Biology. Num Pages: 225 pages, 7 black & white illustrations, 20 colour illustrations, 6 black & white tables, biography. BIC Classification: MJCM. Category: (P) Professional & Vocational. Dimension: 178 x 262 x 19. Weight in Grams: 608. . 2013. 2013th Edition. hardcover. . . . . N° de réf. du vendeur V9781627034807
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