Pyrosequencing: Innovations and Applications - Couverture rigide

Couverture rigide

ISBN 10 : 177361150X ISBN 13 : 9781773611501

Editeur : Delve Publishing, 2017

Synopsis

Pyrosequencing is a DNA sequencing technique that involves sequencing by synthesis, and employs a series of enzymes to detect nucleic acid sequences during the synthesis and enables qualitative analysis of the DNA. A major advantage of this technique is that the labeled primers and nucleotides, as well as gel-electrophoresis is not required. The method requires minimal amounts of samples and gives a high throughput at a lower cost. Besides, the sequence reaction time per base calling is shortened, with higher accuracy, better automation and parallel processing. Pyrosequencing offers a valuable tool for obtaining and analysis of accurate data, identification of new alleles, polymorphic genetic markers, especially Single Nucleotide polymorphisms.The primary purpose of this book is to discuss basics of the Pyrosequencing technology and its key features, including the general sequencing modes, limitations, and future applications. We look forward to feedback by our readers

A propos du livre

À propos de l?auteur

Dr Prerna Pandey, a biotechnologist with several years of wet lab research experience, has worked as a senior research fellow at International Center for Genetic Engineering and Biotechnology, New Delhi. Her field of research in PhD included isolation and molecular characterization of Geminiviruses, genome sequencing, gene annotation, as well as gene silencing using the RNA interference technology. She has published papers in peer-reviewed journals in the field and has a submitted a number of annotated Geminiviral genome sequences in the GenBank, including two novel ones. Prerna has also completed her editing and proof-reading courses from SfEP, UK and now works as a freelance scientific editor, writer and a subject matter expert. She has edited and co-authored several books on a range of topics in Life-Sciences. When Prerna is not busy with her assignments, she loves visiting historical places.

Détails de l'édition

• Éditeur: Delve Publishing
• Date d'édition: 2017
• Langue: anglais
• ISBN 10: 177361150X
• ISBN 13: 9781773611501
• Reliure: Relié
• Nombre de pages: 297
• Éditeur: Pandey Prerna
• Coordonnées du fabricant: non disponible
• Personne responsable: non disponible