Articles liés à PCR
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PCR is the most widely used technique in molecular biology. New PCR variants offering substantial benefits to existing protocols appear on a frequent basis. PCR: Methods Express describes the very latest PCR-based methodologies and approaches to provide the most up-to-date practical advice on how to tackle a broad range of biological problems including: *real time qRT-PCR; *rapid generation of gene targeting constructs; *PCR multiplexes; *PCR-based mutagenesis; *identification of microdeletions and microduplications; *DNA methylation analysis; *forensic genetic DNA typing; *genotyping; *identification of mutations in single cells; *whole genome amplification; *diagnosis of infectious diseases; *inverse PCR-based RFLP. This book is a comprehensive research guide; every chapter discusses the merits and limitations of the available approaches and then provides fully-proven protocols with hints and tips for success. PCR: Methods Express is an essential laboratory manual for researchers in all life science fields and at all levels, from postgraduate student to principal investigator. Contents: 1. An introduction to the polymerase chain reaction (Adrian Moody); 2. Polymerases for PCR (Meg Martel, Simon Baker, Ian Kavanagh and Simon May); 3. A detailed guide to quantitative RT-PCR (Pete Kaiser); 4. Use of quantitative PCR for the detection of genomic microdeletions or microduplications (Simon Hughes, Rosanna Weksberg, Laura Moldovan and Jeremy A. Squire); 5. Robust and unique PCR for single-nucleotide polymorphism genotyping applications (Xiangning Chen); 6. Using PCR and linkage mapping to identify single genes and quantitative trait loci for livestock traits (Jill Maddox, Imke Tammen and Sonja Dominik); 7. PCR restriction fragment length polymorphism analysis for genotyping of single-nucleotide polymorphisms (Simon Hughes); 8. Forensic genetic DNA typing with PCR-based methods (Claus Borsting, Juan J. Sanchez and Niels Morling); 9. Large PCR multiplexes with special reference to forensic single-nucleotide polymorphism typing (Juan J. Sanchez, Claus Borsting and Niels Morling); 10. Pre-implantation genetic diagnosis of monogenic disease: PCR-based methods for the identification of mutations in single cells (Dagan Wells); 11. Rapid generation of gene-targeting constructs (Trevor J. Wilson, Dirk Truman, Antonietta Giudice and Paul Hertzog); 12. Construction of long DNA molecules from multiple fragments using PCR (Nikolai A. Shevchuk and Anton V. Bryksin); 13. Efficient PCR-based mutagenesis method applicable to diverse mutagenesis strategies using type IIs restriction enzyme (Jae-Kyun Ko and Jianjie Ma); 14. Inverse PCR-based restriction fragment length polymorphism for identifying low-level mutations in tumors (G.Mike Makrigiorgos); 15. PCR methods for infectious disease diagnosis (Padmini Ramachandran, Andrew Hardick, Charlotte Gaydos, Samuel Young and Richard Rothman); 16. Use of PCR for DNA methylation analysis (Mario F.Fraga and Manel Esteller); 17. PCR-based methods to determine DNA methylation status at specific CpG sites using methylation-sensitive restriction enzymes (Helmtrud I. Roach and Ko Hashimoto); 18. PCR-based whole genome amplification (Nona Arneson, Simon Hugehs, Richard Houlston and Susan Done); 19. PCR sequencing of human genes for the discovery of DNA sequence variants (Abizar Lakdawalla); List of suppliers; Index. From the Publisher: This title is part of Scion's Methods Express series: a series of books written for busy life science researchers. Each book provides: protocols in step-by-step detail; comprehensive troubleshooting; example data as benchmarks; key references and further reading. Books in the series include: Bioinformatics, Biosensors, Cell Imaging, DNA Microarrays, Expression Systems, Immunohistochemistry, PCR, Proteomics, Whole Genome Amplification.
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- ÉditeurScion Publishing Ltd
- Date d'édition2007
- ISBN 10 1904842291
- ISBN 13 9781904842293
- ReliureRelié
- Numéro d'édition1
- Nombre de pages348
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