Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols - Couverture souple

Till, Bradley J. J.

 
9783319386676: Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Couverture souple
ISBN 10 : 3319386670 ISBN 13 : 9783319386676
Editeur : Springer, 2016

Synopsis

1 Introduction

1.1 Background

1.2 Methods used to isolate genomic DNA from plant tissues

1.3 Methods for the discovery and characterization of induced and natural nucleotide variation in plant genomes

References

2 Health and Safety Considerations

2.1 Guidelines

2.2 Preparation of a home-made chemical spill kit

References

3 Sample Collection and Storage

3.1 Background

3.2 Materials

3.3 Methods

References

4 Low-Cost DNA Extraction

4.1 Materials

4.2 Methods

4.3 Alternative buffers for DNA extraction

References

5 PCR Amplification for Low-Cost Mutation Discovery

5.1 Materials

5.2 Methods

References

6 Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples

6.1 Materials

6.2 Methods

References

7 Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants

7.1 Materials

7.2 Methods

8 Example Data

8.1 Quality of genomic DNA obtained by silica powder-based DNA extraction method

8.2 Quality of genomic DNA obtained by silica powder-based DNA extraction using alternative buffers method

8.3 Example of PCR products using TILLING primers with source genomic DNA from Qiagen kits and low-cost silica method

8.4 Example of low-cost agarose gel based TILLING assays for the discovery of induced point mutations

8.5 Example of enzyme activity recorded from weeds compared to crude celery juice extract

9 Conclusions

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Éditeur
Springer
Date d'édition
2016
Langue
anglais
ISBN 10 
3319386670
ISBN 13 
9783319386676
Reliure
Broché
Nombre de pages
48
Coordonnées du fabricant
non disponible
Personne responsable
CMN Printpool Inh. Mathis Neumann
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Hamburg
Allemagne

Autres éditions populaires du même titre

9783319162584: Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

Edition présentée

ISBN 10 :  3319162586 ISBN 13 :  9783319162584
Editeur : Springer International Publishin..., 2015
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