Comparative Transcriptome Analysis using cDNA–AFLP Technique - Couverture souple

 
9783330001664: Comparative Transcriptome Analysis using cDNA–AFLP Technique
Couverture souple
ISBN 10 : 3330001666 ISBN 13 : 9783330001664
Editeur : LAP LAMBERT Academic Publishing, 2019

Synopsis

An increased understanding of the molecular mechanisms involved in establishment and development of entomopathogenic fungi can be regarded as an important step towards their effective future insect pest management. To this end, I have successfully applied two different PCR-based approaches – cDNA-AFLPs and Quantitative real-time PCR analysis to identify B. bassiana genes putatively involved in overcoming host insect specific barriers. The cDNA-AFLP display proved to be a very powerful transcriptome-wide gene discovery tool in B. bassiana just as with many other organisms in which this approach was used. Without any previous sequence information, it was possible to gain first insights into the molecular processes endowing metabolic versatility to B. bassiana. However, gene knockout experiments have to be conducted to confirm their putative role in various metabolic pathways. Understanding the interconnection between nitrogen metabolism repression and absence of diverse subtilisin like proteases during growth on insect cuticle is pivotal to understand the metabolic versatility of B. bassiana.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

An increased understanding of the molecular mechanisms involved in establishment and development of entomopathogenic fungi can be regarded as an important step towards their effective future insect pest management. To this end, I have successfully applied two different PCR-based approaches – cDNA-AFLPs and Quantitative real-time PCR analysis to identify B. bassiana genes putatively involved in overcoming host insect specific barriers. The cDNA-AFLP display proved to be a very powerful transcriptome-wide gene discovery tool in B. bassiana just as with many other organisms in which this approach was used. Without any previous sequence information, it was possible to gain first insights into the molecular processes endowing metabolic versatility to B. bassiana. However, gene knockout experiments have to be conducted to confirm their putative role in various metabolic pathways. Understanding the interconnection between nitrogen metabolism repression and absence of diverse subtilisin like proteases during growth on insect cuticle is pivotal to understand the metabolic versatility of B. bassiana.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2019
Langue
anglais
ISBN 10 
3330001666
ISBN 13 
9783330001664
Reliure
Broché
Nombre de pages
160
Éditeur
Pathan Akbar Ali Khan
Coordonnées du fabricant
non disponible
Personne responsable
KreGa Onlinehandel GmbH
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