Method Development and Validation for Voriconazole Estimation: RP-HPLC and UV Spectrophotometric Methods in Bulk and Pharmaceutical Formulations - Couverture souple

Srinivasa Rao, Yarguntla; Vara Prasada Rao, K.

Couverture souple

ISBN 10 : 3330051337 ISBN 13 : 9783330051331

Editeur : LAP LAMBERT Academic Publishing, 2024

Synopsis

A rapid, sensitive, and specific RP-HPLC method with UV detection was developed and validated for the determination of voriconazole. Chromatography was performed on an Enable C18 G column (250 × 4.6 mm) in isocratic mode, using a mobile phase of methanol and acetonitrile (60:40, v/v). The flow rate was set at 1 mL/min, and the effluent was monitored at 223 nm. Chromatographic analysis revealed a main peak for voriconazole at a retention time of 2.841 minutes. Methanol was selected as the solvent for the analysis, with the λmax determined to be 223 nm. The method exhibited linearity over a concentration range of 5–30 µg/mL. Validation parameters, including linearity, range, precision, accuracy, limit of detection (LOD), and limit of quantitation (LOQ), were assessed and met the required standards.

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2024
• Langue: anglais
• ISBN 10: 3330051337
• ISBN 13: 9783330051331
• Reliure: Broché
• Nombre de pages: 80
• Coordonnées du fabricant: non disponible
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