Quantitation of Mrna by Polymerase Chain Reaction: Nonradioactive Pcr Methods - Couverture rigide

Kohler, Thomas; Lassner, Dirk; Rost, Anne-Katrin; Thamm, Barbara; Pustowoit, Barbara; Remke, Harald

Couverture rigide

ISBN 10 : 3540591923 ISBN 13 : 9783540591924

Editeur : Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 1995

Synopsis

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.

Détails de l'édition

• Éditeur: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
• Date d'édition: 1995
• Langue: anglais
• ISBN 10: 3540591923
• ISBN 13: 9783540591924
• Reliure: Relié
• Nombre de pages: 186
• Coordonnées du fabricant: non disponible
• Personne responsable: CMN Printpool Inh. Mathis Neumann; https://www.also.com/ec/cms5/en_6000/6000/index.jsp; ; Friedrich-Karl-Str. 9; Hamburg; Allemagne