Articles liés à Quantitation of Mrna by Polymerase Chain Reaction:...

Quantitation of Mrna by Polymerase Chain Reaction: Nonradioactive Pcr Methods - Couverture rigide

Kohler, Thomas; Lassner, Dirk; Rost, Anne-Katrin; Thamm, Barbara; Pustowoit, Barbara; Remke, Harald

 
9783540591924: Quantitation of Mrna by Polymerase Chain Reaction: Nonradioactive Pcr Methods
Couverture rigide
ISBN 10 : 3540591923 ISBN 13 : 9783540591924
Editeur : Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 1995

Synopsis

In this laboratory "cook-book", the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Éditeur
Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Date d'édition
1995
Langue
anglais
ISBN 10 
3540591923
ISBN 13 
9783540591924
Reliure
Relié
Nombre de pages
186
Coordonnées du fabricant
non disponible
Personne responsable
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