Articles liés à PCR: Clinical Diagnostics and Research

PCR: Clinical Diagnostics and Research - Couverture souple

 
9783540594321: PCR: Clinical Diagnostics and Research

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Synopsis

This PCR manual seeks to provide a solid grounding in basic techniques in addition to clinical diagnostic applications. Detailed protocols are given for the use of PCR research and clinical laboratories, backed by information at each step. This second edition includes several new aspects, such as: how to set up a PCR lab and how to control contamination and false negative results; isolation of RNA from clinical samples; sequencing of PCR products; long range PCR; nucleotide analogues in PCR; PCR cloning, RT-RNA PCR, microsatellite PCR, in situ PCR; and single-cell cDNA PCR and application to forensic analysis.

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Présentation de l'éditeur

The "how-to" book for every laboratory using PCR! Complete information aboutPCR procedures, combined with the discussion of contemporary opinions, givesa good overview of this multifaceted method and its applications. Even experienced laboratories will welcome the clear presentation of demanding procedures such as the exact quantification of PCR products or the in-situ PCR. Protocols that have been tried and tested provide swift support for troubleshooting all sorts of procedural problems. Indeed, many examples of problem areas found in diagnostically oriented PCR are fully described. Furthermore, the most important lab PCR equipment as well as the pros and cons of single individual reaction components which are necessary for performing PCR reactions are discussed. The book is clearly organized to give an introduction to each technique, brief background information, step-by-step instructions for carrying out the procedure and additional comments.

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Autres éditions populaires du même titre

9783642774942: PCR: Clinical Diagnostics and Research: Clinical Diagnostics and Research (Springer Lab Manuals)

Edition présentée

ISBN 10 :  3642774946 ISBN 13 :  9783642774942
Editeur : Springer, 2012
Couverture souple