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9783540618959: Microinjection and Transgenesis: Strategies and Protocols

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ISBN 10 : 3540618953 ISBN 13 : 9783540618959

Editeur : Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 1997

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The most potent technique to study cell function is the specific tranfer of genetic material into a single cell. Several functions can than be analyzed in the resulting transgenic cell or organism. The method of microinjection and transgenesis is currently used in many cell biological laboratories.

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�diteur: Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Date d'�dition: 1997
Langue: anglais
ISBN 10: 3540618953
ISBN 13: 9783540618959
Reliure: Broch�
Nombre de pages: 633
�diteur: Cid-Arregui Angel, Garcia-Carranca Alejandro
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Microinjection and Transgenesis: Strategies and Protocols

Alejandro Garcia-Carranca et Angel Cid-Arregui

Edit� par Springer-Verlag Berlin and Heidelberg GmbH & Co. K, 1997

ISBN 10 : 3540618953 ISBN 13 : 9783540618959

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Softcover. Etat : Bon. L�g�res traces d'usure sur la couverture. Edition 1997. Ammareal reverse jusqu'� 15% du prix net de cet article � des organisations caritatives. ENGLISH DESCRIPTION Book Condition: Used, Good. Slight signs of wear on the cover. Edition 1997. Ammareal gives back up to 15% of this item's net price to charity organizations. N� de r�f. du vendeur D-733-447

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Microinjection and Transgenesis

Angel Cid-Arregui

Edit� par Springer, Springer Nov 1997, 1997

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics. 636 pp. Englisch. N� de r�f. du vendeur 9783540618959

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Microinjection and Transgenesis

Cid-Arregui, A.|Garcia-Carranca, A.

Edit� par Springer Berlin Heidelberg, 1997

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Kartoniert / Broschiert. Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. A practical tool presenting the latest techniques developed in renowned laboratoriesEasy to follow step-by-step protocolsBackground information and full practical details (materials needed, exact recipes, troubleshooting guide, typical results, list. N� de r�f. du vendeur 4895801

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Microinjection and Transgenesis

Angel Cid-Arregui

Edit� par Springer, Springer Nov 1997, 1997

ISBN 10 : 3540618953 ISBN 13 : 9783540618959

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -The most potent technique to study cell function is the specific tranfer of genetic material into a single cell. Several functions can than be analyzed in the resulting transgenic cell or organism. The method of microinjection and transgenesis is currently used in many cell biological laboratories.Springer-Verlag KG, Sachsenplatz 4-6, 1201 Wien 636 pp. Englisch. N� de r�f. du vendeur 9783540618959

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Microinjection and Transgenesis | Strategies and Protocols

Angel Cid-Arregui (u. a.)

Edit� par Springer, 1997

ISBN 10 : 3540618953 ISBN 13 : 9783540618959

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Microinjection and Transgenesis : Strategies and Protocols

Alejandro Garcia-Carranca

Edit� par Springer Berlin Heidelberg, 1997

ISBN 10 : 3540618953 ISBN 13 : 9783540618959

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Vendeur : AHA-BUCH GmbH, Einbeck, Allemagne

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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics. N� de r�f. du vendeur 9783540618959

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Microinjection and Transgenesis: Strategies and Protocols (Springer Lab Manuals)

Edit� par Springer, 1997

ISBN 10 : 3540618953 ISBN 13 : 9783540618959

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