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Manipulation of Genetic Information in StudyingPlant Performance - Couverture souple

Mansour, Ahmed

 
9783639004205: Manipulation of Genetic Information in StudyingPlant Performance
Couverture souple
ISBN 10 : 3639004205 ISBN 13 : 9783639004205
Editeur : VDM Verlag Dr. Müller, 2008

Synopsis

Plant tolerance to heat stress proved to be entirely dependent on thesignaling flow of information by which the plant can sense the changes in itssurrounding environment and signal its genes to respond by producingspecial proteins to protect it-self. Any changes or manipulations in thissignaling flow of information will presumably lead to a modification in thegenetic expression inside the plant cells, consequently, changing plantperformance. Understanding these signaling events in response to heat mayhelp us to produce heat tolerant plants capable to stand high temperaturestress. In the present investigation, the results showed that a heat activatedMAP kinase cascade, involving heat activated MAP kinase (HAMK), playedan essential role in heat shock gene expression in tobacco BY-2 cells. Inorder to determine if heat activation of HAMK involved additional pathwaysof signaling we studied the upstream regulation of HAMK, includingmembrane fluidization and reorganization of cytoskeleton. The activation ofHAMK and accumulation of heat responsive HSFs, HSP70 and HSP27proteins were used as end-point markers in these experiments. It isconcluded that the heat shock response, as measured by HAMK activationand heat shock proteins accumulation required PKC activation, membranefluidization and reorganization of the cytoskeleton. A comparativebioinformatic explanation of similarities between tobacco heat shock genesand their counterparts in different organisms revealed a high degree ofevolutionary conservation in the corresponding domains, indicating similarfunction in different species.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Plant tolerance to heat stress proved to be entirely dependent on thesignaling flow of information by which the plant can sense the changes in itssurrounding environment and signal its genes to respond by producingspecial proteins to protect it-self. Any changes or manipulations in thissignaling flow of information will presumably lead to a modification in thegenetic expression inside the plant cells, consequently, changing plantperformance. Understanding these signaling events in response to heat mayhelp us to produce heat tolerant plants capable to stand high temperaturestress. In the present investigation, the results showed that a heat activatedMAP kinase cascade, involving heat activated MAP kinase (HAMK), playedan essential role in heat shock gene expression in tobacco BY-2 cells. Inorder to determine if heat activation of HAMK involved additional pathwaysof signaling we studied the upstream regulation of HAMK, includingmembrane fluidization and reorganization of cytoskeleton. The activation ofHAMK and accumulation of heat responsive HSFs, HSP70 and HSP27proteins were used as end-point markers in these experiments. It isconcluded that the heat shock response, as measured by HAMK activationand heat shock proteins accumulation required PKC activation, membranefluidization and reorganization of the cytoskeleton. A comparativebioinformatic explanation of similarities between tobacco heat shock genesand their counterparts in different organisms revealed a high degree ofevolutionary conservation in the corresponding domains, indicating similarfunction in different species.

Biographie de l'auteur

Dr. Ahmed Mansour is an Egyptian scientist who obtained his Ph.D. degree in (Plant Signal transduction) in Faculty of agriculture, Zagazig University, Egypt, in collaboration with McGill University, Canada, where he spends two years of full experimental work, and his dissertation research entitled (Manipulation of Genetic information in studying plant performance). He have been invited and participated in many international scientific meetings and conferences in molecular genetics around the world. He also has many scientific publications in the field of Molecular Genetics and Bioninformatics.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
VDM Verlag Dr. Müller
Date d'édition
2008
Langue
anglais
ISBN 10 
3639004205
ISBN 13 
9783639004205
Reliure
Broché
Nombre de pages
112
Coordonnées du fabricant
non disponible
Personne responsable
non disponible