Articles liés à Fullerene Derivatives: And Their Application in Bioanalytics

Fullerene Derivatives: And Their Application in Bioanalytics - Couverture souple

Vallant, Rainer

 
9783639005400: Fullerene Derivatives: And Their Application in Bioanalytics
Couverture souple
ISBN 10 : 3639005406 ISBN 13 : 9783639005400
Editeur : VDM Verlag Dr. Müller, 2008

Synopsis

Fullerene derivatives, such as dioctadecyl methano fullerene, fullerenoacetic acid, and IDA-[60]-fullerene were prepared and subjected to a comprehensive characterization study including protein binding properties and capacity. These fullerene derivatives were successfully applied as material-enhanced laser desorption/ionization (MELDI) carrier materials. It is shown that diverse functionalities result in characteristic human serum peak patterns (m/z 2000-20 000) which vary in terms of signal intensity as well as number of detectable masses. In addition, the fullerene derivatives clearly provided differences in the low molecular weight mass region (m/z 1000-4000) after elution of the adsorbed serum constituents. Monolithic, high-resolution capillary columns, were employed for eluate separation and target spotting. Thus, serum compounds were successfully fractionated and subjected to MS/MS analysis. This contribution, hence, proposes a new 'top-down' strategy for proteome research enabling protein profiling as well as biomarker identification in the low-mass range using selective enrichment, high-resolution separation, and offline MALDI-MS/MS evaluation.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Fullerene derivatives, such as dioctadecyl methano fullerene, fullerenoacetic acid, and IDA-[60]-fullerene were prepared and subjected to a comprehensive characterization study including protein binding properties and capacity. These fullerene derivatives were successfully applied as material-enhanced laser desorption/ionization (MELDI) carrier materials. It is shown that diverse functionalities result in characteristic human serum peak patterns (m/z 2000-20 000) which vary in terms of signal intensity as well as number of detectable masses. In addition, the fullerene derivatives clearly provided differences in the low molecular weight mass region (m/z 1000-4000) after elution of the adsorbed serum constituents. Monolithic, high-resolution capillary columns, were employed for eluate separation and target spotting. Thus, serum compounds were successfully fractionated and subjected to MS/MS analysis. This contribution, hence, proposes a new 'top-down' strategy for proteome research enabling protein profiling as well as biomarker identification in the low-mass range using selective enrichment, high-resolution separation, and offline MALDI-MS/MS evaluation.

Biographie de l'auteur

Rainer Vallant, born 1977 in Johannesburg, South Africa; from 2005 PhD-Student at the Institute of Analytical Chemistry and Radiochemistry, University Innsbruck, under the supervision of Prof. Dr. Günther Bonn. The aim of his work is based on the synthesis and modification of nano-structured materials and their further bioanalytical application.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
VDM Verlag Dr. Müller
Date d'édition
2008
Langue
anglais
ISBN 10 
3639005406
ISBN 13 
9783639005400
Reliure
Broché
Nombre de pages
84
Coordonnées du fabricant
non disponible
Personne responsable
non disponible