The Role of Complexin in Regulated Exocytosis: A Study Shows that the SNARE-binding Protein Complexin is a Positive Regulator of Vesicle Priming in Fast, Calcium-triggered Exocytosis - Couverture souple

Cai, Haijiang

 
9783639014396: The Role of Complexin in Regulated Exocytosis: A Study Shows that the SNARE-binding Protein Complexin is a Positive Regulator of Vesicle Priming in Fast, Calcium-triggered Exocytosis
Couverture souple
ISBN 10 : 3639014391 ISBN 13 : 9783639014396
Editeur : VDM Verlag Dr. Müller, 2008

Synopsis

This work has studied the role of complexin in regulated exocytosis, a key mechanism underlies hormone and neurotransmitter release and understanding of which will help to design therapeutic interventions in a lot of neuronal diseases or endocrine diseases.The work here systematically dissects the vesicle stages leading up to exocytosis using a knockout-rescue strategy in a mammalian model system. The work shows that adrenal chromaffin cells from CPX II knockout mice exhibit a markedly diminished readily releasable vesicle pool, while showing no change in the kinetics of fusion pore dilation or morphological vesicle docking. Overexpression of wildtype CPX II ? but not of SNARE-binding-deficient mutants -- restores the size of the readily releasable pool in knockout cells, and in wildtype cells it markedly enlarges the readily releasable pool. These results suggest that CPXs prime vesicles for exocytosis and, therefore, are positive regulators of Ca2+-triggered exocytosis.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

This work has studied the role of complexin in regulated exocytosis, a key mechanism underlies hormone and neurotransmitter release and understanding of which will help to design therapeutic interventions in a lot of neuronal diseases or endocrine diseases.The work here systematically dissects the vesicle stages leading up to exocytosis using a knockout-rescue strategy in a mammalian model system. The work shows that adrenal chromaffin cells from CPX II knockout mice exhibit a markedly diminished readily releasable vesicle pool, while showing no change in the kinetics of fusion pore dilation or morphological vesicle docking. Overexpression of wildtype CPX II ? but not of SNARE-binding-deficient mutants -- restores the size of the readily releasable pool in knockout cells, and in wildtype cells it markedly enlarges the readily releasable pool. These results suggest that CPXs prime vesicles for exocytosis and, therefore, are positive regulators of Ca2+-triggered exocytosis.

Biographie de l'auteur

Dr. Haijiang Cai is currenly a postdoc fellow in California Institute of Technology, USA. He received his B.Sc. from the University of Science and Technology of China and his Ph.D. from the University of Southern California, USA. His research interests include synaptic transmission and neural circuits.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
VDM Verlag Dr. Müller
Date d'édition
2008
Langue
anglais
ISBN 10 
3639014391
ISBN 13 
9783639014396
Reliure
Broché
Nombre de pages
132
Coordonnées du fabricant
non disponible
Personne responsable
non disponible