MOLECULAR INTERACTIONS AT THE CHEMISTRY-BIOLOGY INTERFACE: The Design of Interfacial Biomaterials and the Modulation of Galectin¿3 Aggregation With Multivalent Saccharide Ligands - Couverture souple

Parise, James

 
9783639097993: MOLECULAR INTERACTIONS AT THE CHEMISTRY-BIOLOGY INTERFACE: The Design of Interfacial Biomaterials and the Modulation of Galectin¿3 Aggregation With Multivalent Saccharide Ligands
Couverture souple
ISBN 10 : 3639097998 ISBN 13 : 9783639097993
Editeur : VDM Verlag, 2008

Synopsis

Myriad biological processes are governed by chemical reactions that occur on cell surfaces. In the first part of this work, a novel approach to direct biological processes that occur at the surface of artificial materials is described. A multi-faceted strategy has been developed to construct ¿interfacial biomaterials¿ that mediate specific biological processes via increased cellular adhesion to medically-relevant materials. A phage display selection strategy was developed to identify peptides that show increased affinity for natural and artificial substrates. In the second part, the molecular basis of protein¿carbohydrate binding is considered. Ligands displaying lactose epitopes were constructed to investigate their aggregative properties towards full¿length and truncated galectin¿3. ITC studies with full¿length protein shows a greater than two¿fold enhancement in affinity for the bivalent ligand compared to monovalent ligand. This behavior demonstrates that protein-protein interactions and aggregation are the primary cause for affinity increases observed with polyvalent saccharide ligands, and unambiguously establishes a molecular basis for the cluster glycoside effect.

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À propos de l?auteur

James A. Parise Jr, PhD, is an Instructor and the Manager of the Organic Chemistry Laboratories at Duke University in Durham, North Carolina. He received his PhD in organic chemistry at Duke University in 2007 under the direction of Eric Toone and did his postdoctoral work with David Lawrence at the University of North Carolina at Chapel Hill.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
VDM Verlag
Date d'édition
2008
Langue
anglais
ISBN 10 
3639097998
ISBN 13 
9783639097993
Reliure
Broché
Nombre de pages
160
Coordonnées du fabricant
non disponible
Personne responsable
non disponible