OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER: A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS - Couverture souple
Synopsis
This dissertation presents a novel technique of delivering membrane impermeable molecules into single living cells with the assistance of femtosecond (fs) near-infrared (NIR) laser pulses. This technique promises several major advantages, namely, very high transfection efficiency, high cell survival rate (¿100%) and fully preserved cell viabilities. In this work, the system for fs NIR opto-injection was designed and built. Successful fs NIR opto-injection has been performed on several cell systems including single mammalian cells, marine animal eggs, and human cancer cells cultured in a tissue-like environment. The connections between laser parameters and cell responses were explored. Dye uptake rate of the target cells was observed to depend on incident laser intensity. Pore size was found dependent on incident laser intensity. The conclusion was made that laser-induced breakdown and plasma-induced ablation in cell membrane are the physical principles that govern the process of fs NIR opto-injection.
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OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER: A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS
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VDM Verlag Dr. Müller, 2008
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ISBN 13 : 9783639098921
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OPTOINJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS
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OPTOINJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS
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OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER: A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS
Edité par
VDM Verlag Dr. Müller, 2008
ISBN 10 : 3639098927
ISBN 13 : 9783639098921
Neuf
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OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER: A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS
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VDM Verlag Dr. M�ller 2008-11-24, 2008
ISBN 10 : 3639098927
ISBN 13 : 9783639098921
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OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER
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OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER
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VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2008
ISBN 10 : 3639098927
ISBN 13 : 9783639098921
Neuf
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Edité par
VDM Verlag Dr. Mueller Aktiengesellschaft & Co. KG, 2008
ISBN 10 : 3639098927
ISBN 13 : 9783639098921
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OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER | A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS
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Taschenbuch. Etat : Neu. OPTO-INJECTION INTO SINGLE LIVING CELLS BY FEMTOSECOND NIR LASER | A NOVEL TECHNIQUE OF DELIVERING MATERIALS INTO LIVEING CELLS | Cheng Peng | Taschenbuch | Kartoniert / Broschiert | Englisch | 2008 | VDM Verlag Dr. Müller | EAN 9783639098921 | Verantwortliche Person für die EU: OmniScriptum GmbH & Co. KG, Bahnhofstr. 28, 66111 Saarbrücken, info[at]akademikerverlag[dot]de | Anbieter: preigu. N° de réf. du vendeur 101698487
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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This dissertation presents a novel technique ofdelivering membrane impermeable molecules into singleliving cells with the assistance of femtosecond (fs)near-infrared (NIR) laser pulses. This techniquepromises several major advantages, namely, very hightransfection efficiency, high cell survival rate( 100%) and fully preserved cell viabilities. In this work, the system for fs NIR opto-injectionwas designed and built. Successful fs NIRopto-injection has been performed on several cellsystems including single mammalian cells, marineanimal eggs, and human cancer cells cultured in atissue-like environment. The connections betweenlaser parameters and cell responses were explored.Dye uptake rate of the target cells was observed todepend on incident laser intensity. Pore size wasfound dependent on incident laser intensity. Theconclusion was made that laser-induced breakdown andplasma-induced ablation in cell membrane are thephysical principles that govern the process of fs NIRopto-injection. N° de réf. du vendeur 9783639098921
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