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A Green Fluorescent Protein-Based Purification System for Plant Cells: Optimizing Yield and Recovery of Recombinant Proteins from Plants - Couverture souple

Peckham, Gabriel

 
9783639126426: A Green Fluorescent Protein-Based Purification System for Plant Cells: Optimizing Yield and Recovery of Recombinant Proteins from Plants
Couverture souple
ISBN 10 : 3639126424 ISBN 13 : 9783639126426
Editeur : VDM Verlag Dr. Müller, 2009

Synopsis

Purifying recombinant proteins from plants is often met with difficulties due to the complexity of plant cell extracts. Issues include high levels of protease activity, tannin and phenolic reactivity and hydrophobic aggregation. Difficulties in extraction and purification are major hurdles preventing the routine use of plants and plant suspension cells as bioreactors. Reporter molecules, such as green fluorescent protein, are often used to monitor recombinant protein expression levels. Therefore, a purification strategy was developed taking advantage of this commonly used reporter molecule. Although previous methods have been developed for purifying GFP, these strategies often included exposure to protein-denaturing solvents or required gel filtration and protein concentrating steps. This work details a strategy that optimizes yield and purity without jeopardizing the integrity of the fusion protein.

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Présentation de l'éditeur

Purifying recombinant proteins from plants is often met with difficulties due to the complexity of plant cell extracts. Issues include high levels of protease activity, tannin and phenolic reactivity and hydrophobic aggregation. Difficulties in extraction and purification are major hurdles preventing the routine use of plants and plant suspension cells as bioreactors. Reporter molecules, such as green fluorescent protein, are often used to monitor recombinant protein expression levels. Therefore, a purification strategy was developed taking advantage of this commonly used reporter molecule. Although previous methods have been developed for purifying GFP, these strategies often included exposure to protein-denaturing solvents or required gel filtration and protein concentrating steps. This work details a strategy that optimizes yield and purity without jeopardizing the integrity of the fusion protein.

Biographie de l'auteur

Gabriel is currently a postdoc developing field tests for plant pathogens at the University of Hawaii. These tests combine the best of serological and DNA detection methods. Most of his time outside the lab is spent with his son Jaden. Hobbies include tennis, basketball, disc golf, scuba diving and poker.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
VDM Verlag Dr. Müller
Date d'édition
2009
Langue
anglais
ISBN 10 
3639126424
ISBN 13 
9783639126426
Reliure
Broché
Nombre de pages
148
Coordonnées du fabricant
non disponible
Personne responsable
non disponible