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High Resolution Imaging of Polytene Chromosomes: Illustrated Protocols of New Method and Computer Vision Perspectives - Couverture souple

Novikov, Dmitri

 
9783639129243: High Resolution Imaging of Polytene Chromosomes: Illustrated Protocols of New Method and Computer Vision Perspectives
Couverture souple
ISBN 10 : 3639129245 ISBN 13 : 9783639129243
Editeur : VDM Verlag Dr. Müller, 2009

Synopsis

The exceptional organization of polytene chromosomes has made fruit fly Drosophila melanogaster a premier model organism for visualization of genes. A major barrier to full exploitation of polytene chromosome cytology has been the difficulty in producing good chromosome preparations. A new, economical procedure using high pressure and other modifications to overcome this barrier is presented. Light microscopy images of such slides compared to electron microscopy images for the first time show similar or superior detail. Results from typical staining applications, including immunofluorescence and fluorescence in situ hybridization are illustrated, and optimized protocols for light and electron microscopy presented. Also included are notes on the general architecture of polytene chromosomes. Improved chromosome images prompted collaborative development of computer vision applications for automated recognition and analysis of structural patterns. These methods should be helpful for researchers and students using the classical polytene chromosome model as well as to all scientists exploring wonderful possibilities that computer vision opens to modern microscopy.

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Présentation de l'éditeur

The exceptional organization of polytene chromosomes has made fruit fly Drosophila melanogaster a premier model organism for visualization of genes. A major barrier to full exploitation of polytene chromosome cytology has been the difficulty in producing good chromosome preparations. A new, economical procedure using high pressure and other modifications to overcome this barrier is presented. Light microscopy images of such slides compared to electron microscopy images for the first time show similar or superior detail. Results from typical staining applications, including immunofluorescence and fluorescence in situ hybridization are illustrated, and optimized protocols for light and electron microscopy presented. Also included are notes on the general architecture of polytene chromosomes. Improved chromosome images prompted collaborative development of computer vision applications for automated recognition and analysis of structural patterns. These methods should be helpful for researchers and students using the classical polytene chromosome model as well as to all scientists exploring wonderful possibilities that computer vision opens to modern microscopy.

Biographie de l'auteur

Dmitri V. Novikov, Ph.D., University of Illinois. Director of Microscopy Facility of the School of Molecular and Cellular Biology at the University of Illinois.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
VDM Verlag Dr. Müller
Date d'édition
2009
Langue
anglais
ISBN 10 
3639129245
ISBN 13 
9783639129243
Reliure
Broché
Nombre de pages
96
Coordonnées du fabricant
non disponible
Personne responsable
non disponible