Articles liés à Detection of VLPs by ISEM in transgenic grapevine:...

Detection of VLPs by ISEM in transgenic grapevine: Is ISEM suitable for the Detection of GFLV - VLPs in transgenic grapevines? - Couverture souple

Gottschamel, Johanna

 
9783639236910: Detection of VLPs by ISEM in transgenic grapevine: Is ISEM suitable for the Detection of GFLV - VLPs in transgenic grapevines?
Couverture souple
ISBN 10 : 3639236912 ISBN 13 : 9783639236910
Editeur : VDM Verlag Dr. Müller, 2010

Synopsis

Grapevine fanleaf virus (GFLV) is one of the most destructive and wide-spread viral diseases affecting grapevine (Bovey et al. 1980). Since virus disease control with conventional methods is very difficult, major efforts are made towards resistance breeding. Coat-protein-mediated resistance (CPmR) has demonstrated to confer a high level of resistance in herbaceous model plants (Beachy et al. 1990; Pacot-Hiriat et al. 1999) and is a promising strategy to obtain virus-resistance in perennial plants like grapevine using a pathogen derived gene. In order to produce resistant grapevines not only an efficient protection, but also environmental safety aspects have to be considered (Gölles et al. 2000). The main purpose of this work is to answer the question whether truncated coat proteins maintain the capacity of self-assembly, i.e. if empty capsids occur in transgenic plants. For this approach ISEM was chosen as the method of analysis, because of its direct and rapid results. It could be clearly demonstrated that ISEM is a suitable method for VLP detection in CP-transgenic grapevines and that it could be recommended as a continuous standard monitoring technique for field experiments.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Grapevine fanleaf virus (GFLV) is one of the most destructive and wide-spread viral diseases affecting grapevine (Bovey et al. 1980). Since virus disease control with conventional methods is very difficult, major efforts are made towards resistance breeding. Coat-protein-mediated resistance (CPmR) has demonstrated to confer a high level of resistance in herbaceous model plants (Beachy et al. 1990; Pacot-Hiriat et al. 1999) and is a promising strategy to obtain virus-resistance in perennial plants like grapevine using a pathogen derived gene. In order to produce resistant grapevines not only an efficient protection, but also environmental safety aspects have to be considered (Gölles et al. 2000). The main purpose of this work is to answer the question whether truncated coat proteins maintain the capacity of self-assembly, i.e. if empty capsids occur in transgenic plants. For this approach ISEM was chosen as the method of analysis, because of its direct and rapid results. It could be clearly demonstrated that ISEM is a suitable method for VLP detection in CP-transgenic grapevines and that it could be recommended as a continuous standard monitoring technique for field experiments.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
VDM Verlag Dr. Müller
Date d'édition
2010
Langue
anglais
ISBN 10 
3639236912
ISBN 13 
9783639236910
Reliure
Broché
Nombre de pages
88
Coordonnées du fabricant
non disponible
Personne responsable
non disponible