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Receptor Specificity and Cellular Entry of Human Rhinoviruses: Receptor recognition and virus entry pathways - Couverture souple

Khan, Abdul Ghafoor

 
9783639282337: Receptor Specificity and Cellular Entry of Human Rhinoviruses: Receptor recognition and virus entry pathways
Couverture souple
ISBN 10 : 3639282337 ISBN 13 : 9783639282337
Editeur : VDM Verlag Dr. Müller, 2010

Synopsis

Human rhinoviruses (HRVs), the major cause of the common cold, are classified into two groups on the basis of receptor specificity. Minor group HRVs use members of the low-density lipoprotein receptor (LDLR) super-family while major group types bind to intercellular adhesion molecule-1 (ICAM-1). Here we provide evidence that there is a increasing trend for the involvement of heparan sulfate (HS) proteoglycans as an alternate receptor. Concerning virus entry, where minor group HRVs depend on clathrin-mediated endocytosis, the pathway of major group HRVs and that of HS-binding viruses is largely unknown. Combining immunofluorescence technique with electron microscopy, dominant negative mutants and pharmacological inhibitor assays, we demonstrate that the major group virus (HRV14) enters via dynamin- independent macropinocytosis into RD-ICAM cells whereas virus entry into RD cells via HS exhibits similar characteristics with respect to co- localization with endocytic markers and pharmacological inhibitor profiles. However, this virus entry is dependent on functional dynamin and viruses accumulate in comparatively larger vesicular structures.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Human rhinoviruses (HRVs), the major cause of the common cold, are classified into two groups on the basis of receptor specificity. Minor group HRVs use members of the low-density lipoprotein receptor (LDLR) super-family while major group types bind to intercellular adhesion molecule-1 (ICAM-1). Here we provide evidence that there is a increasing trend for the involvement of heparan sulfate (HS) proteoglycans as an alternate receptor. Concerning virus entry, where minor group HRVs depend on clathrin-mediated endocytosis, the pathway of major group HRVs and that of HS-binding viruses is largely unknown. Combining immunofluorescence technique with electron microscopy, dominant negative mutants and pharmacological inhibitor assays, we demonstrate that the major group virus (HRV14) enters via dynamin- independent macropinocytosis into RD-ICAM cells whereas virus entry into RD cells via HS exhibits similar characteristics with respect to co- localization with endocytic markers and pharmacological inhibitor profiles. However, this virus entry is dependent on functional dynamin and viruses accumulate in comparatively larger vesicular structures.

Biographie de l'auteur

was born in Jhang, Pakistan, did his MPhil degree in Molecular Biology and moved to Austria for PhD. He completed his PhD in 2009 with distinction from Vienna Biocenter, University of Vienna. He started his job as Assistant Professor at NUST Center of Virology and Immunology (NCVI), National University of Sciences and Technology, Islamabad.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
VDM Verlag Dr. Müller
Date d'édition
2010
Langue
anglais
ISBN 10 
3639282337
ISBN 13 
9783639282337
Reliure
Broché
Nombre de pages
140
Coordonnées du fabricant
non disponible
Personne responsable
non disponible