Advanced Biophysical Approaches to Nano-Bio-Sensing: Potential Environmental and Biomedical Applications Based on p53-Azurin Interaction - Couverture souple

Domenici, Fabio

 
9783639706161: Advanced Biophysical Approaches to Nano-Bio-Sensing: Potential Environmental and Biomedical Applications Based on p53-Azurin Interaction
Couverture souple
ISBN 10 : 3639706161 ISBN 13 : 9783639706161
Editeur : Scholars' Press, 2013

Synopsis

Nano-bio-sensing based on plasmonics is now entrenching in environmental and biomedical fields, thus opening to the possibility of providing new anticancer strategies. This work is devoted to the exploration of the interaction between p53 and Az. p53 is a valuable indicator of cancer cells and can be recognized by Az even enhancing its tumour suppressor activity. The analytical potential of plasmonics has been addressed in the attempt to overcome detection limits of Raman and Absorbance spectroscopies. Surface-enhanced Raman spectroscopy, also combined with AFM, provided an approach to improve the detection limit of the tumour marker p53 recognized by Az. This lays the foundations for the development of innovative experimental methods for an ultrasensitive, easy and rapid screening of many different molecules of environmental and biomedical relevance. Surface Plasmon Resonance technology allowed to accurately find binding kinetics features on Az-p53 recognition and its subsequent ability to interfere with p53-Mdm2 binding, the latter playing a key role in lowering the intracellular stability of p53. The work should be mainly useful to applied physicists, biochemists and biologists.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Nano-bio-sensing based on plasmonics is now entrenching in environmental and biomedical fields, thus opening to the possibility of providing new anticancer strategies. This work is devoted to the exploration of the interaction between p53 and Az. p53 is a valuable indicator of cancer cells and can be recognized by Az even enhancing its tumour suppressor activity. The analytical potential of plasmonics has been addressed in the attempt to overcome detection limits of Raman and Absorbance spectroscopies. Surface-enhanced Raman spectroscopy, also combined with AFM, provided an approach to improve the detection limit of the tumour marker p53 recognized by Az. This lays the foundations for the development of innovative experimental methods for an ultrasensitive, easy and rapid screening of many different molecules of environmental and biomedical relevance. Surface Plasmon Resonance technology allowed to accurately find binding kinetics features on Az-p53 recognition and its subsequent ability to interfere with p53-Mdm2 binding, the latter playing a key role in lowering the intracellular stability of p53. The work should be mainly useful to applied physicists, biochemists and biologists.

Biographie de l'auteur

Fabio Domenici, PhD: Studied Physics of Biosystems at Sapienza University of Rome and at the Biophysics and Nanoscience Centre CNISM, Tuscia University of Viterbo. He presently conducts research activities in the field of nano-bio-sensing and drug delivery at the Dept. of Physics of Sapienza University.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Scholars' Press
Date d'édition
2013
Langue
anglais
ISBN 10 
3639706161
ISBN 13 
9783639706161
Reliure
Broché
Nombre de pages
132
Coordonnées du fabricant
non disponible
Personne responsable
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