Histologic Examination of Host-Parasite Interactions Fundulus grandis: Examining the cellular immune response in the gulf killifish to nematode parasites found within the mesenteries - Couverture souple

Marsh, Marlee

 
9783639766592: Histologic Examination of Host-Parasite Interactions Fundulus grandis: Examining the cellular immune response in the gulf killifish to nematode parasites found within the mesenteries
Couverture souple
ISBN 10 : 3639766598 ISBN 13 : 9783639766592
Editeur : Scholars' Press, 2015

Synopsis

Little is known about the immunobiology of fish responses to parasitic infections. The aim of this study was to describe a model for fish-nematode interactions on the immunological front, and to develop new reagents for the study of this model. Gulf killifish, Fundulus grandis, were collected over two seasons from MS, USA, and examined for endoparasites, particularly the larval nematode Eustrongylides ignotus. A baseline study was preformed to determine the prevalence, intensity, and histologic features of E. ignotus infection in F. grandis. In addition, two monoclonal antibodies, (mAb) M24-2 and 2C11, were characterized for their use in this project, as well as for general applications in fish immunobiology. mAb M24-2 was developed and found to recognize a ~ 14.5 kD protein in serum from all fish tested, and recognizes hen egg lysozyme (HEL). mAb 2C11 was developed and determined to recognize a ~17 kD protein found in fish cell lysates, but in not plasma. It was determined that mAb 2C11 recognizes granule content of a small population of highly granulated cells, most likely eosinophilic granular cells (EGCs). Like mAb M24-2, mAb 2C11 was found to be cross reactive in other fish.

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Présentation de l'éditeur

Little is known about the immunobiology of fish responses to parasitic infections. The aim of this study was to describe a model for fish-nematode interactions on the immunological front, and to develop new reagents for the study of this model. Gulf killifish, Fundulus grandis, were collected over two seasons from MS, USA, and examined for endoparasites, particularly the larval nematode Eustrongylides ignotus. A baseline study was preformed to determine the prevalence, intensity, and histologic features of E. ignotus infection in F. grandis. In addition, two monoclonal antibodies, (mAb) M24-2 and 2C11, were characterized for their use in this project, as well as for general applications in fish immunobiology. mAb M24-2 was developed and found to recognize a ~ 14.5 kD protein in serum from all fish tested, and recognizes hen egg lysozyme (HEL). mAb 2C11 was developed and determined to recognize a ~17 kD protein found in fish cell lysates, but in not plasma. It was determined that mAb 2C11 recognizes granule content of a small population of highly granulated cells, most likely eosinophilic granular cells (EGCs). Like mAb M24-2, mAb 2C11 was found to be cross reactive in other fish.

Biographie de l'auteur

Dr. Marlee Marsh is currently an assistant professor of Biology at Columbia College in SC. Dr. Marsh’s research interests include comparative immunology with a focus on immunoparasitology, comparative histology and hematology. She has an active research program involving undergraduates. Her students have recognized her for her teaching.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
Scholars' Press
Date d'édition
2015
Langue
anglais
ISBN 10 
3639766598
ISBN 13 
9783639766592
Reliure
Broché
Nombre de pages
120
Coordonnées du fabricant
non disponible
Personne responsable
non disponible