Proteins at Solid-Liquid Interfaces: Principles And Practice - Couverture souple

9783642069130: Proteins at Solid-Liquid Interfaces: Principles And Practice

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ISBN 10 : 3642069134 ISBN 13 : 9783642069130

Editeur : Springer, 2010

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This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Presented here are methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS). Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight-secondary ion mass spectrometry (TOF-SIMS). A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this invaluable book.

Les informations fournies dans la section � Synopsis � peuvent faire r�f�rence � une autre �dition de ce titre.

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�diteur: Springer
Date d'�dition: 2010
Langue: anglais
ISBN 10: 3642069134
ISBN 13: 9783642069130
Reliure: Broch�
Nombre de pages: 348
�diteur: D�jardin Philippe
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Poststr. 9
Darmstadt
64293
Allemagne

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Proteins at Solid-Liquid Interfaces (eng)

D�jardin, Philippe

Edit� par Springer, 2010

ISBN 10 : 3642069134 ISBN 13 : 9783642069130

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Proteins at Solid-Liquid Interfaces: Principles And Practice

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Proteins at Solid-Liquid Interfaces

Philippe D�jardin

Edit� par Springer Berlin Heidelberg Nov 2010, 2010

ISBN 10 : 3642069134 ISBN 13 : 9783642069130

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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The adsorption of proteins at interfaces plays a role in many elds, such as health, food, environment and analysis. Fundamental aspects are useful when considering applications. We focus here especially on solid liquid interfaces and present a few fundamental studies regarding adsorption - netics and conformational changes, and examples of applications to sensors and membranes. The rst part is dedicated to fundamental studies performed using - tical waveguide lightmode spectroscopy, as an example of a technique that has the advantage of not requiring labelled proteins, but is limited to s- ci c supports. Conversely, the radiolabelling of proteins, which has the disadvantage of any labelling process, allows application to any kind of s- faces. As proteins bear both positive and negative charges, we can expect thein uenceofanelectric eldnormaltothe interfaceonthe pack- ing order at interfaces. The re ning of data treatment may also lead to the determination of useful structural parameters. The balance between protein surface and protein protein interactions is a key point for the - scription of the structure at high coverage of the surface. Electrokinetic methods, like measurement of the streaming potential, may be helpful in the electrical characterisation of the interfacial layer facing the solution. The second part includes different bench techniques that were dev- oped to improve the sensitivity of the characterisation of the orientation and structure of the proteins at interfaces: dual polarisation interferometry and total internal re ection ellipsometry are such recent examples. 348 pp. Englisch. N� de r�f. du vendeur 9783642069130

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Proteins at Solid-Liquid Interfaces

D�jardin, Philippe

Edit� par Springer Berlin Heidelberg, 2010

ISBN 10 : 3642069134 ISBN 13 : 9783642069130

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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Describes fundamental aspects of protein adsorption to surfacesPresents methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopyIncludes a model study of fibronectin at polymer sur. N� de r�f. du vendeur 5046001

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Proteins at Solid-Liquid Interfaces

Philippe D�jardin

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Proteins at Solid-Liquid Interfaces

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Proteins at Solid-Liquid Interfaces

Philippe D�jardin

Edit� par Springer Berlin Heidelberg, Springer Nov 2010, 2010

ISBN 10 : 3642069134 ISBN 13 : 9783642069130

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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -The adsorption of proteins at interfaces plays a role in many elds, such as health, food, environment and analysis. Fundamental aspects are useful when considering applications. We focus here especially on solid�liquid interfaces and present a few fundamental studies regarding adsorption - netics and conformational changes, and examples of applications to sensors and membranes. The rst part is dedicated to fundamental studies performed using - tical waveguide lightmode spectroscopy, as an example of a technique that has the advantage of not requiring labelled proteins, but is limited to s- ci c supports. Conversely, the radiolabelling of proteins, which has the disadvantage of any labelling process, allows application to any kind of s- faces. As proteins bear both positive and negative charges, we can expect thein uenceofanelectric eldnormaltothe interfaceonthe pack- ing order at interfaces. The re ning of data treatment may also lead to the determination of useful structural parameters. The balance between protein�surface and protein�protein interactions is a key point for the - scription of the structure at high coverage of the surface. Electrokinetic methods, like measurement of the streaming potential, may be helpful in the electrical characterisation of the interfacial layer facing the solution. The second part includes different bench techniques that were dev- oped to improve the sensitivity of the characterisation of the orientation and structure of the proteins at interfaces: dual polarisation interferometry and total internal re ection ellipsometry are such recent examples.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 348 pp. Englisch. N� de r�f. du vendeur 9783642069130

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Proteins at Solid-Liquid Interfaces

Philippe D�jardin

Edit� par Springer Berlin Heidelberg, 2010

ISBN 10 : 3642069134 ISBN 13 : 9783642069130

Neuf Taschenbuch

Vendeur : AHA-BUCH GmbH, Einbeck, Allemagne

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Taschenbuch. Etat : Neu. Druck auf Anfrage Neuware - Printed after ordering - The adsorption of proteins at interfaces plays a role in many elds, such as health, food, environment and analysis. Fundamental aspects are useful when considering applications. We focus here especially on solid liquid interfaces and present a few fundamental studies regarding adsorption - netics and conformational changes, and examples of applications to sensors and membranes. The rst part is dedicated to fundamental studies performed using - tical waveguide lightmode spectroscopy, as an example of a technique that has the advantage of not requiring labelled proteins, but is limited to s- ci c supports. Conversely, the radiolabelling of proteins, which has the disadvantage of any labelling process, allows application to any kind of s- faces. As proteins bear both positive and negative charges, we can expect thein uenceofanelectric eldnormaltothe interfaceonthe pack- ing order at interfaces. The re ning of data treatment may also lead to the determination of useful structural parameters. The balance between protein surface and protein protein interactions is a key point for the - scription of the structure at high coverage of the surface. Electrokinetic methods, like measurement of the streaming potential, may be helpful in the electrical characterisation of the interfacial layer facing the solution. The second part includes different bench techniques that were dev- oped to improve the sensitivity of the characterisation of the orientation and structure of the proteins at interfaces: dual polarisation interferometry and total internal re ection ellipsometry are such recent examples. N� de r�f. du vendeur 9783642069130

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