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Molecular Study of Rice Plant Rhizobacteria of Indo-Nepal Border: A research work for improved yield of crops - Couverture souple

Shrivastava, Umesh P.

 
9783659132094: Molecular Study of Rice Plant Rhizobacteria of Indo-Nepal Border: A research work for improved yield of crops
Couverture souple
ISBN 10 : 3659132098 ISBN 13 : 9783659132094
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

The present investigation deals with the isolation and characterization of rhizospheric diazotrophic bacteria from the rice fields of border region of India and Nepal. Molecular diversity among different isolates has been established by PCR-based techniques, restriction analysis of 16S rRNA gene, gene sequencing and DGGE. All the isolates were tested for plant growth promoting characteristics (IAA production, phosphate solubilization, and siderophore production) along with N2 fixation. A novel, rapid and simple method for the screening of IAA production plate assay technique was developed during this study. Our findings show the following significant achievements: i) novel and rapid method of detection of IAA, ii) P solubilization and ACCD activity by Microbacterium (ECI-12A), iii) Siderophore production by Agrobacterium (BN-2A) under salinity stress seems to be the first report. iv) Early flowering by inoculation of BN-2A and v) Species of Agrobacterium, Klebsiella, Serratia, Pseudomonas, Azotobacter and Microbacterium etc are predominantly present in the rhizosphere of rice plants of Indo-Nepal border region.

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Présentation de l'éditeur

The present investigation deals with the isolation and characterization of rhizospheric diazotrophic bacteria from the rice fields of border region of India and Nepal. Molecular diversity among different isolates has been established by PCR-based techniques, restriction analysis of 16S rRNA gene, gene sequencing and DGGE. All the isolates were tested for plant growth promoting characteristics (IAA production, phosphate solubilization, and siderophore production) along with N2 fixation. A novel, rapid and simple method for the screening of IAA production plate assay technique was developed during this study. Our findings show the following significant achievements: i) novel and rapid method of detection of IAA, ii) P solubilization and ACCD activity by Microbacterium (ECI-12A), iii) Siderophore production by Agrobacterium (BN-2A) under salinity stress seems to be the first report. iv) Early flowering by inoculation of BN-2A and v) Species of Agrobacterium, Klebsiella, Serratia, Pseudomonas, Azotobacter and Microbacterium etc are predominantly present in the rhizosphere of rice plants of Indo-Nepal border region.

Biographie de l'auteur

Umesh Prasad Shrivastava, Ph.D.: Awarded Ph.D. (Biotechnology) at Banaras Hindu University, Varanasi, India. Associate Professor, Department of Botany, Tribhuvan University, T.R.M. Campus, Birgunj, Nepal. He has published many research papers and book chapters in national and international journals/books in his 23 years of teaching carrier.

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Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659132098
ISBN 13 
9783659132094
Reliure
Broché
Nombre de pages
200
Coordonnées du fabricant
non disponible
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