Development of Agrobacterium Mediated Transformation System for Tomato: Transgenic Development - Couverture souple

Pawar, Bhausaheb; Jadhav, Ashok; Kale, Anil

 
9783659134715: Development of Agrobacterium Mediated Transformation System for Tomato: Transgenic Development
Couverture souple
ISBN 10 : 3659134716 ISBN 13 : 9783659134715
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

This book present the work that has immense application in genetic manipulation of tomato to enhance productivity through increasing pest and disease resistance, environmental stress tolerance and to study gene function and regulation. The absence of highly efficient regeneration and transformation procedure is major obstacle in application of gene transformation technology to economically important tomato cultivars. Improved protocol for Agrobacterium mediated transformation of tomato cultivar; “Dhanshree” was developed by optimizing various parameters that affect transformation efficiency. In the present investigation Agrobacterium strain EHA 105 Harboring a pBI 121: gus gene construct was used. Cotyledons proved to be better for transformation as compared to hypocotyls and leaf explants. The best transformation efficiency was obtained with 7-14 days old explants. This transformation protocol proved to be simple, repeatable does not require any callusing phase, and may become general means of transforming tomato.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

This book present the work that has immense application in genetic manipulation of tomato to enhance productivity through increasing pest and disease resistance, environmental stress tolerance and to study gene function and regulation. The absence of highly efficient regeneration and transformation procedure is major obstacle in application of gene transformation technology to economically important tomato cultivars. Improved protocol for Agrobacterium mediated transformation of tomato cultivar; “Dhanshree” was developed by optimizing various parameters that affect transformation efficiency. In the present investigation Agrobacterium strain EHA 105 Harboring a pBI 121: gus gene construct was used. Cotyledons proved to be better for transformation as compared to hypocotyls and leaf explants. The best transformation efficiency was obtained with 7-14 days old explants. This transformation protocol proved to be simple, repeatable does not require any callusing phase, and may become general means of transforming tomato.

Biographie de l'auteur

Mr. Bhausaheb Dnyanoba Pawar is Research Assistant in State Level Biotechnology Centre, Department of Agril Botany at Mahatma Phule Krishi Vidyapeeth Rahuri, Maharashtra, India. His area of specialization is transgenic development.He did his graduation and post graduation in Agril. Biotechnology from Mahatma Phule Krishi Vidyapeeth Rahuri.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659134716
ISBN 13 
9783659134715
Reliure
Broché
Nombre de pages
84
Coordonnées du fabricant
non disponible
Personne responsable
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