Lipstatin: Fermentative Production: Fermentative Production and Downstream processing of Lipstatin - Couverture souple

Kumar, Maushmi; Verma, Vikas

 
9783659148040: Lipstatin: Fermentative Production: Fermentative Production and Downstream processing of Lipstatin
Couverture souple
ISBN 10 : 3659148040 ISBN 13 : 9783659148040
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

Streptomyces toxytricini a Gram-positive bacterium belonging to the genus Streptomyces is producer of the pancreatic lipase inhibitor lipstatin,of which the antiobesity drug orlistat is a derivative.The study has been done for optimization of seed media and fermentation media using Plackett-Burman method for increasing the yield of the strain selected.Purification was done on silica gel column and identification of lipstatin molecule using sophisticated techniques such as IR, NMR, and mass spectroscopy. The presence and identification was also confirmed using TLC and HPLC method. This study also compares the impurity profile and the percentage purity of the lipstatin. The optimized media gave a titer of Lipstatin (1980 mg/ml) which is comparable to highest producing benchmarks (2400 mg/ml), but it is quite economical in a way that this work eliminates the use of L-Leucine as media component. The further increase in yield can be tried by different UV, chemical mutation methods in future.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Streptomyces toxytricini a Gram-positive bacterium belonging to the genus Streptomyces is producer of the pancreatic lipase inhibitor lipstatin,of which the antiobesity drug orlistat is a derivative.The study has been done for optimization of seed media and fermentation media using Plackett-Burman method for increasing the yield of the strain selected.Purification was done on silica gel column and identification of lipstatin molecule using sophisticated techniques such as IR, NMR, and mass spectroscopy. The presence and identification was also confirmed using TLC and HPLC method. This study also compares the impurity profile and the percentage purity of the lipstatin. The optimized media gave a titer of Lipstatin (1980 mg/ml) which is comparable to highest producing benchmarks (2400 mg/ml), but it is quite economical in a way that this work eliminates the use of L-Leucine as media component. The further increase in yield can be tried by different UV, chemical mutation methods in future.

Biographie de l'auteur

Maushmi S KumarShobhaben Pratapbhai Patel School of Pharmacy and Technology Management,NMIMS University, Mumbai, INDIAB.Pharm from VNS Inst of Pharmacy in 2003 ,M.Tech in Bio process Technology from Institute of Chemical Technology, Mumbai in 2006.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659148040
ISBN 13 
9783659148040
Reliure
Broché
Nombre de pages
80
Coordonnées du fabricant
non disponible
Personne responsable
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