Somatic Embryogenesis and Genetic Transformation in Sugarcane: Plant Regeneration through Somatic Embryogenesis in Sugarcane and its Transformation - Couverture souple

Roy, Mohashweta; Hossain, Monzur; Islam, Rafiul

 
9783659153884: Somatic Embryogenesis and Genetic Transformation in Sugarcane: Plant Regeneration through Somatic Embryogenesis in Sugarcane and its Transformation
Couverture souple
ISBN 10 : 3659153885 ISBN 13 : 9783659153884
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

An efficient protocol for somatic embryogenesis and subsequent plant regeneration was developed for sugarcane variety Isd-16 using leaf sheath explants of 3, 6 and 12-months-old field grown plants. Explants from 6-month-old plant showed the best response and produced highest percentage of calli on MS + 3.0 mg/l 2,4-D. L-proline 25mg/l significantly enhanced somatic embryogenesis. The embryos germinated well on half-strength MS and developed into plantlets. Somatic embryo derived plants under field condition showed considerable variation in morphological, agronomical and biochemical characters. For genetic transformation the calli were co-cultivated with A. tumefaciens strain LBA4404 harboring a binary plasmid pCAMBIA1303 containing hpt (hygromycin phosphotransferase) gene as a selectable marker and a β-glucuronidase (gus) reporter gene in the T-DNA region. The transient expression of gus in hygromycin resistant calli and regenerated plants was confirmed by GUS flurometric assay. PCR analysis of genomic DNA from regenerated plants revealed that the hpt gene was integrated in the transgenic plants.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

An efficient protocol for somatic embryogenesis and subsequent plant regeneration was developed for sugarcane variety Isd-16 using leaf sheath explants of 3, 6 and 12-months-old field grown plants. Explants from 6-month-old plant showed the best response and produced highest percentage of calli on MS + 3.0 mg/l 2,4-D. L-proline 25mg/l significantly enhanced somatic embryogenesis. The embryos germinated well on half-strength MS and developed into plantlets. Somatic embryo derived plants under field condition showed considerable variation in morphological, agronomical and biochemical characters. For genetic transformation the calli were co-cultivated with A. tumefaciens strain LBA4404 harboring a binary plasmid pCAMBIA1303 containing hpt (hygromycin phosphotransferase) gene as a selectable marker and a β-glucuronidase (gus) reporter gene in the T-DNA region. The transient expression of gus in hygromycin resistant calli and regenerated plants was confirmed by GUS flurometric assay. PCR analysis of genomic DNA from regenerated plants revealed that the hpt gene was integrated in the transgenic plants.

Biographie de l'auteur

Mohashweta Roy, PhD: Studied Botany at Chittagong Univ. Asst Prof of Botany, Chittagong Govt. Women's College, Bangladesh.Monzur Hossain, PhD: Studied Botany at Rajshahi Univ, Rajshahi, Bangladesh. Prof of Botany, Rajshahi Univ.Rafiul Islam, MPhil, PhD: Studied Botany at Rajshahi Univ, Rajshahi, Bangladesh. Prof of Botany, Rajshahi Univ.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659153885
ISBN 13 
9783659153884
Reliure
Broché
Nombre de pages
164
Coordonnées du fabricant
non disponible
Personne responsable
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