Articles liés à Detection of Tuberculosis By Polymerase Chain Reaction...

Detection of Tuberculosis By Polymerase Chain Reaction (PCR): Detection of Pulmonary Tuberculosis by Polymerase Chain Reaction Using Rapid and Efficient DNA Extraction Method - Couverture souple

Dey, Hymonti; Debnath, Sudip

 
9783659161445: Detection of Tuberculosis By Polymerase Chain Reaction (PCR): Detection of Pulmonary Tuberculosis by Polymerase Chain Reaction Using Rapid and Efficient DNA Extraction Method
Couverture souple
ISBN 10 : 3659161446 ISBN 13 : 9783659161445
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

The polymerase chain reaction (PCR) has recently been investigated for detecting Mycobacterium tuberculosis in clinical specimens and appears to have significant potential as a rapid, sensitive, and specific assay for diagnosing infections caused by this organism. One of the remaining issues regarding the use of this technique is the extraction of DNA from clinical material prior to performing the PCR. It requires the use of DNA extraction method that can efficiently lyse mycobacterial cells and recover small amounts of DNA. The method of heating the sample in a boiling water bath to break down the bacterial cell wall and to release the DNA was compared with that of enzymatic lysis of bacteria and then phenol-chloroform extraction of DNA (Modified chemical method). Modified chemical method was better than just boiling the sample to remove DNA for amplification with PCR. In this study 140 sputum samples were collected from suspected prisoners at Dhaka Central Jail. All of those samples were prepared by two DNA extraction methods and examined by PCR, and the results were compared with the results of acid-fast stained smears and cultures.

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Présentation de l'éditeur

The polymerase chain reaction (PCR) has recently been investigated for detecting Mycobacterium tuberculosis in clinical specimens and appears to have significant potential as a rapid, sensitive, and specific assay for diagnosing infections caused by this organism. One of the remaining issues regarding the use of this technique is the extraction of DNA from clinical material prior to performing the PCR. It requires the use of DNA extraction method that can efficiently lyse mycobacterial cells and recover small amounts of DNA. The method of heating the sample in a boiling water bath to break down the bacterial cell wall and to release the DNA was compared with that of enzymatic lysis of bacteria and then phenol-chloroform extraction of DNA (Modified chemical method). Modified chemical method was better than just boiling the sample to remove DNA for amplification with PCR. In this study 140 sputum samples were collected from suspected prisoners at Dhaka Central Jail. All of those samples were prepared by two DNA extraction methods and examined by PCR, and the results were compared with the results of acid-fast stained smears and cultures.

Biographie de l'auteur

Hymonti Dey, is a promising Research Officer presently working in the Tuberculosis Laboratory, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B). She did her MS in Biochemistry and Molecular Biology from University of Dhaka, Bangladesh. She has significant skills on PCR technology.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659161446
ISBN 13 
9783659161445
Reliure
Broché
Nombre de pages
104
Coordonnées du fabricant
non disponible
Personne responsable
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