Les cellules souches ont la capacité unique de produire plus d'elles-mêmes (c'est-à-dire à « auto-renouveler ») et à générer des cellules tissulaires spécialisées. Afin d'utiliser cette double capacité pour la médecine régénérative ou les modèles tissulaires in vitro, nous devons comprendre les mécanismes qui régulent le comportement des cellules souches au niveau d'une seule cellule. Cependant, les méthodologies in vitro actuelles pour étudier les cellules individuelles sont soit limitées par leur débit ou ne peuvent pas suivre efficacement les cellules souches uniques sur de longues périodes de temps et ensuite distinguer leur destin. Cette thèse a abordé ces lacunes en développant des dispositifs miniaturisés pour manipuler et analyser des cellules souches vivantes uniques. Les composants présentés permettent i) la culture de cellules souches simples, ii) le suivi des cellules par analyse d'image automatisée et iii) la séparation physique des cellules filles de sorte que iv) les cellules filles d'une seule souche cellulaire peuvent être analysées individuellement par ex. RT-PCR. De tels dispositifs multifonctionnels de laboratoire sur puce ouvriront la porte à des paradigmes expérimentaux qui n'étaient pas imaginables auparavant et fourniront des informations uniques sur la biologie des cellules souches.
Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.
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Taschenbuch. Etat : Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Stem cells have the unique ability to produce more of themselves (i.e. to self-renew ) and to generate specialized tissue cells. In order to use this dual ability for regenerative medicine or in vitro tissue models, we have to understand the mechanisms that regulate stem cell behavior at a single cell level. However, current in vitro methodologies to study single cells are either limited by their throughput or cannot efficiently track single stem cells over long time periods and then still distinguish their fate. This thesis addressed these shortcomings by developing miniaturized devices to handle and analyze single live stem cells. The presented components allow i) the culture of single stem cells, ii) the tracking of the cells by automated image analysis and iii) the physical separation of daughter cells such that iv) the daughter cells of a single cell stem can be analyzed individually by e.g. RT-PCR. Such multi-functional lab-on-a-chip devices will open the door to experimental paradigms that have previously not been imaginable and will provide unique insights into stem cell biology. 176 pp. Englisch. N° de réf. du vendeur 9783659161889
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Etat : New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Kobel Stefan A.Stefan Kobel studied biology and chemistry at ETH Zurich, Switzerland, and completed his PhD in bioengineering at EPF Lausanne, Switzerland. He is currently a group leader at Roche Diagnostics, where he works on blood . N° de réf. du vendeur 5136039
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Etat : Like New. Used - Like New. Book is new and unread but may have minor shelf wear. Your purchase helps support Sri Lankan Children's Charity 'The Rainbow Centre'. Our donations to The Rainbow Centre have helped provide an education and a safe haven to hundreds of children who live in appalling conditions. N° de réf. du vendeur Z1-U-026-01399
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Taschenbuch. Etat : Neu. Microtechnologies to fate-map single stem cells and their progeny | How to culture, track and individually analyze single stem cells and their progeny with micro-devices | Stefan A. Kobel | Taschenbuch | 176 S. | Englisch | 2013 | LAP LAMBERT Academic Publishing | EAN 9783659161889 | Verantwortliche Person für die EU: BoD - Books on Demand, In de Tarpen 42, 22848 Norderstedt, info[at]bod[dot]de | Anbieter: preigu. N° de réf. du vendeur 106342189
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Taschenbuch. Etat : Neu. This item is printed on demand - Print on Demand Titel. Neuware -Stem cells have the unique ability to produce more of themselves (i.e. to 'self-renew') and to generate specialized tissue cells. In order to use this dual ability for regenerative medicine or in vitro tissue models, we have to understand the mechanisms that regulate stem cell behavior at a single cell level. However, current in vitro methodologies to study single cells are either limited by their throughput or cannot efficiently track single stem cells over long time periods and then still distinguish their fate. This thesis addressed these shortcomings by developing miniaturized devices to handle and analyze single live stem cells. The presented components allow i) the culture of single stem cells, ii) the tracking of the cells by automated image analysis and iii) the physical separation of daughter cells such that iv) the daughter cells of a single cell stem can be analyzed individually by e.g. RT-PCR. Such multi-functional lab-on-a-chip devices will open the door to experimental paradigms that have previously not been imaginable and will provide unique insights into stem cell biology.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 176 pp. Englisch. N° de réf. du vendeur 9783659161889
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Taschenbuch. Etat : Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Stem cells have the unique ability to produce more of themselves (i.e. to self-renew ) and to generate specialized tissue cells. In order to use this dual ability for regenerative medicine or in vitro tissue models, we have to understand the mechanisms that regulate stem cell behavior at a single cell level. However, current in vitro methodologies to study single cells are either limited by their throughput or cannot efficiently track single stem cells over long time periods and then still distinguish their fate. This thesis addressed these shortcomings by developing miniaturized devices to handle and analyze single live stem cells. The presented components allow i) the culture of single stem cells, ii) the tracking of the cells by automated image analysis and iii) the physical separation of daughter cells such that iv) the daughter cells of a single cell stem can be analyzed individually by e.g. RT-PCR. Such multi-functional lab-on-a-chip devices will open the door to experimental paradigms that have previously not been imaginable and will provide unique insights into stem cell biology. N° de réf. du vendeur 9783659161889
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