Articles liés à Enzymatic Synthesis of Modified DNA by PCR and its...

Enzymatic Synthesis of Modified DNA by PCR and its Application: A Study on Synthesis and Applications of Modified DNA - Couverture souple

Masud, Mohammad Mehedi

 
9783659188435: Enzymatic Synthesis of Modified DNA by PCR and its Application: A Study on Synthesis and Applications of Modified DNA
Couverture souple
ISBN 10 : 3659188433 ISBN 13 : 9783659188435
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

Thymidine analogue bearing a methyl ester at the C5 position were synthesized. Thermophilic family B DNA polymerases accepted these analogues as substrate to form the corresponding PCR product. Modified DNA containing this analogue were prepared by PCR with KOD Dash DNA polymerase and were further post-modified to form the corresponding derivatized DNA. These DNA could act as a template for PCR and could accept natural TTP or modified analogue as substrate. The thymidine analogue with an amino-linker was also reacted with fluorescein isothiocyanate, yielding a new analogue which was used as a substrate for PCR to get the corresponding fluorescent labeled DNA. Finally, a novel modified DNA aptamer for sialyllactose that is ubiquitous component of the cell surface responsible for the infections of several viruses was produced by using SELEX method with strong binding ability.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Thymidine analogue bearing a methyl ester at the C5 position were synthesized. Thermophilic family B DNA polymerases accepted these analogues as substrate to form the corresponding PCR product. Modified DNA containing this analogue were prepared by PCR with KOD Dash DNA polymerase and were further post-modified to form the corresponding derivatized DNA. These DNA could act as a template for PCR and could accept natural TTP or modified analogue as substrate. The thymidine analogue with an amino-linker was also reacted with fluorescein isothiocyanate, yielding a new analogue which was used as a substrate for PCR to get the corresponding fluorescent labeled DNA. Finally, a novel modified DNA aptamer for sialyllactose that is ubiquitous component of the cell surface responsible for the infections of several viruses was produced by using SELEX method with strong binding ability.

Biographie de l'auteur

Dr. Masud is working as a Professor for the Faculty of Pharmacy, University of Dhaka, Bangladesh. He obtained his B.Pharm (Hons.) and M. Pharm from University of Dhaka; PhD from Gunma University, Japan. He also worked as a TBRF Post-Doctoral research Fellow at Gunma University. His research interest is in Nucleic Acids and Medicinal Chemistry.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659188433
ISBN 13 
9783659188435
Reliure
Broché
Nombre de pages
124
Coordonnées du fabricant
non disponible
Personne responsable
KreGa Onlinehandel GmbH
4950000000000
info@krega-onlinehandel.de

Birkenweg 31
Brakel
33034
Allemagne