Articles liés à RNA interference (RNAi) mediated Tomato leaf curl virus...

RNA interference (RNAi) mediated Tomato leaf curl virus gene silencing: Development of RNAi based transgenic resistance in tomato by silencing ToLCV replicase gene - Couverture souple

Ramesh, S. V.; Praveen, Shelly

 
9783659194559: RNA interference (RNAi) mediated Tomato leaf curl virus gene silencing: Development of RNAi based transgenic resistance in tomato by silencing ToLCV replicase gene
Couverture souple
ISBN 10 : 3659194557 ISBN 13 : 9783659194559
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

RNA interference (RNAi) is an evolutionarily conserved, homology dependent gene silencing mechanism; an area of upfront basic research, further gaining importance in applications like development of virus resistant transgenics. To explore the possibility of transgenic resistance to the Tomato leaf curl virus,a constraint in tomato production, sub-region of viral replicase gene (truncated rep) was chosen as target for RNA based silencing. In silico analysis of rep gene with siRNA design algorithms and appraisal of rep mRNA, disclosed potent siRNAs and the accessibility of mRNA for gene silencing. Four different RNAi constructs viz., antisense T-rep and three hairpin RNA constructs, were developed and tomato transformed to obtain transformants. Transformation and regeneration of ToLCV infected leaves resulted in regeneration of recovered plants. Interestingly, tomato transformants showed phenotypic anomalies, attributed to the off-target silencing by siRNAs. Thus the book caters to the plant molecular biologists and virologists working on the arena of gene silencing with information on basic criteria for target region selection, construct design strategies and silencing studies.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

RNA interference (RNAi) is an evolutionarily conserved, homology dependent gene silencing mechanism; an area of upfront basic research, further gaining importance in applications like development of virus resistant transgenics. To explore the possibility of transgenic resistance to the Tomato leaf curl virus,a constraint in tomato production, sub-region of viral replicase gene (truncated rep) was chosen as target for RNA based silencing. In silico analysis of rep gene with siRNA design algorithms and appraisal of rep mRNA, disclosed potent siRNAs and the accessibility of mRNA for gene silencing. Four different RNAi constructs viz., antisense T-rep and three hairpin RNA constructs, were developed and tomato transformed to obtain transformants. Transformation and regeneration of ToLCV infected leaves resulted in regeneration of recovered plants. Interestingly, tomato transformants showed phenotypic anomalies, attributed to the off-target silencing by siRNAs. Thus the book caters to the plant molecular biologists and virologists working on the arena of gene silencing with information on basic criteria for target region selection, construct design strategies and silencing studies.

Biographie de l'auteur

Ramesh S. V., Ph.D, received doctorate in Plant Biochemistry from Indian Agricultural Research Institute, New Delhi. Currently working as Scientist (Biotechnology) employing RNAi on soybean improvement, at Directorate of soybean Research (DSR), ICAR, Indore. Dr. Shelly Praveen, FNASc, contributing in the arena of Plant Molecular Virology at IARI.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659194557
ISBN 13 
9783659194559
Reliure
Broché
Nombre de pages
164
Coordonnées du fabricant
non disponible
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