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Molecular Detection and Quantification of BCR-ABL gene: Molecular detection - Couverture souple

Dhahi, Maysaa A. R.

 
9783659202926: Molecular Detection and Quantification of BCR-ABL gene: Molecular detection
Couverture souple
ISBN 10 : 3659202924 ISBN 13 : 9783659202926
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

This study was designed as a try to apply molecular, conventional cytogenetic, and immunological techniques as conformational diagnosis of bcr-abl gene and protein, and also to follow up chronic myeloid leukemia patients (CML) treated with imatinb mesylate (IM) for assessment of response at different IM treatment durations. Molecular screening for the presence of bcr-abl gene in 34 CML patients and negative controls were done using Multiplex-Single Step-Reverse Transcriptase-Polymerase Chain Reaction. The results showed that all CML patients were positive for bcr-abl gene while all the others were negative for this gene. For assessment of molecular response to IM, quantification of bcr-abl gene in CML patients were done using Real Time-Reverse Transcriptase-Polymerase Chain Reaction-Taqman.CML patients, who show signs of treatment non-response or relapse, were screened for T315I mutation using Allele Specific oligonucleotides. The results showed that this mutation dose not detected in any of those patients.In conclusion, obligatory complementary tests that should be performed for any CML patient who start to receive IM, in order to assess the full respons to therapy.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

This study was designed as a try to apply molecular, conventional cytogenetic, and immunological techniques as conformational diagnosis of bcr-abl gene and protein, and also to follow up chronic myeloid leukemia patients (CML) treated with imatinb mesylate (IM) for assessment of response at different IM treatment durations. Molecular screening for the presence of bcr-abl gene in 34 CML patients and negative controls were done using Multiplex-Single Step-Reverse Transcriptase-Polymerase Chain Reaction. The results showed that all CML patients were positive for bcr-abl gene while all the others were negative for this gene. For assessment of molecular response to IM, quantification of bcr-abl gene in CML patients were done using Real Time-Reverse Transcriptase-Polymerase Chain Reaction-Taqman.CML patients, who show signs of treatment non-response or relapse, were screened for T315I mutation using Allele Specific oligonucleotides. The results showed that this mutation dose not detected in any of those patients.In conclusion, obligatory complementary tests that should be performed for any CML patient who start to receive IM, in order to assess the full respons to therapy.

Biographie de l'auteur

Name: Maysaa Abdul Razzaq Dhahi/ Nationality: Iraq/ Qualification: BSc in Microbiology-College of Science. MSc in Biotechnology (Molecular Biology),College of Science- Baghdad University. PhD in Microbiology(Molecular Biology),College of Medicine, Al-Nahrain University

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659202924
ISBN 13 
9783659202926
Reliure
Broché
Nombre de pages
220
Coordonnées du fabricant
non disponible
Personne responsable
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