Isolation & Mol Characterization of Shiga toxin Producing E.coli O157: Isolation and molecular characterization of Shiga toxin producing e. Coli o157 from raw milk and beef - Couverture souple

Shahzad, Khawar Ali; Muhammad, Khushi

 
9783659203954: Isolation & Mol Characterization of Shiga toxin Producing E.coli O157: Isolation and molecular characterization of Shiga toxin producing e. Coli o157 from raw milk and beef
Couverture souple
ISBN 10 : 3659203955 ISBN 13 : 9783659203954
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

Escherichia coli is normal inhabitant of all the animals and human beings. Its non-sorbitol fermenting biotype (SNF) was detectable in buffalo (90 percent), cattle (80 percent) or rarely in sheep (20 percent) and goat (30 percent). However, SNF E. coli were un-detectable in droppings of rural chickens and feces of donkeys. The SNF E. coli was detected in 100, 92, 71 and 80 percent of the market milk samples and 100, 83, 83 and 53 percent beef samples from Multan, Sandha, Wagha and Sheikhupura Roads, of Lahore city, respectively. However, SNF E. coli was not detected from freshly aseptically collected milk and beef samples but was detectable in over all 96 percent of market raw milk and 82 percent market beef samples. White colored colonies of each positive sample on Sorbitol MacConkey’s Agar (SMA) contained gram negative, rods with round ends non spore former. Each of the isolates was sorbitol non fermenter, lactose fermenter, indole positive, Methyl Red positive, Voges Prauskaur negative and citrate negative. However, each of such isolates showed green metallic sheen on Eosin Methylene Blue (EMB) agar. Each of the isolate was further characterized using polymerase chain reaction

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Présentation de l'éditeur

Escherichia coli is normal inhabitant of all the animals and human beings. Its non-sorbitol fermenting biotype (SNF) was detectable in buffalo (90 percent), cattle (80 percent) or rarely in sheep (20 percent) and goat (30 percent). However, SNF E. coli were un-detectable in droppings of rural chickens and feces of donkeys. The SNF E. coli was detected in 100, 92, 71 and 80 percent of the market milk samples and 100, 83, 83 and 53 percent beef samples from Multan, Sandha, Wagha and Sheikhupura Roads, of Lahore city, respectively. However, SNF E. coli was not detected from freshly aseptically collected milk and beef samples but was detectable in over all 96 percent of market raw milk and 82 percent market beef samples. White colored colonies of each positive sample on Sorbitol MacConkey’s Agar (SMA) contained gram negative, rods with round ends non spore former. Each of the isolates was sorbitol non fermenter, lactose fermenter, indole positive, Methyl Red positive, Voges Prauskaur negative and citrate negative. However, each of such isolates showed green metallic sheen on Eosin Methylene Blue (EMB) agar. Each of the isolate was further characterized using polymerase chain reaction

Biographie de l'auteur

My scientific expertise are molecular isolation, identification and characterization of different bilogically and industrially important strains. Cuerrect interests are biochemical analysis of different by-products obtained from different food samples.

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Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659203955
ISBN 13 
9783659203954
Reliure
Broché
Nombre de pages
88
Coordonnées du fabricant
non disponible
Personne responsable
KreGa Onlinehandel GmbH
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