Drought-responsive transcript(s) profiling from the leaves of soybean: Isolation and characterization of drought responsive transcript(s) in Soybean - Couverture souple

Roy, Priti; Ramesh, S.V.

 
9783659212482: Drought-responsive transcript(s) profiling from the leaves of soybean: Isolation and characterization of drought responsive transcript(s) in Soybean
Couverture souple
ISBN 10 : 3659212482 ISBN 13 : 9783659212482
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

Drought responsive genes or transcription factors are important components of any genetic engineering or breeding approach to enhance drought tolerance trait of a crop.Water-deficit stress was imposed on 30 days old seedlings of soybean cultivars Hardee and NRC7.Drought associated physiological parameter like relative water content revealed that water status of the stressed plants is low. Similarly studies on membrane damage disclosed plants under moisture-deficit stress exhibited higher electrolytic leakages, hence greater membrane damage.Total RNA isolated from both the control and stressed plant leaves of cultivars Hardee and NRC-7 was subjected to cDNA synthesis using 3 different anchored primers and thus mRNA population is sub-divided in to 3 different cDNA populations.DDRTPCR using the same anchored primers in combination with eight different arbitrary primers exhibited the transcript profiling of cultivars under moisture deficit stress.The transcript profiling was resolved in denaturing sequencing gel PAGE followed by silver staining so as to identify the differentially expressed transcripts.The book sheds light on drought responsive molecular mechanism of soybean.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Drought responsive genes or transcription factors are important components of any genetic engineering or breeding approach to enhance drought tolerance trait of a crop.Water-deficit stress was imposed on 30 days old seedlings of soybean cultivars Hardee and NRC7.Drought associated physiological parameter like relative water content revealed that water status of the stressed plants is low. Similarly studies on membrane damage disclosed plants under moisture-deficit stress exhibited higher electrolytic leakages, hence greater membrane damage.Total RNA isolated from both the control and stressed plant leaves of cultivars Hardee and NRC-7 was subjected to cDNA synthesis using 3 different anchored primers and thus mRNA population is sub-divided in to 3 different cDNA populations.DDRTPCR using the same anchored primers in combination with eight different arbitrary primers exhibited the transcript profiling of cultivars under moisture deficit stress.The transcript profiling was resolved in denaturing sequencing gel PAGE followed by silver staining so as to identify the differentially expressed transcripts.The book sheds light on drought responsive molecular mechanism of soybean.

Biographie de l'auteur

Priti Roy received MSc in Biotechnology from Pandit Ravishankar Shukla University Raipur (C.G.);Ramesh SV,Ph.D,working as Scientist (Biotechnology)on soybean improvement at DSR,Indian Council Of Agricultural Research(ICAR)-Indore (M.P.) obtained his doctorate in Plant Biochemistry from Indian Agricultural Research Institute-New Delhi.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
3659212482
ISBN 13 
9783659212482
Reliure
Broché
Nombre de pages
108
Coordonnées du fabricant
non disponible
Personne responsable
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