Development and Production of Humanized Monoclonal Antibody: Development, Expression and Characterization of Humanized Anti-C2 Monoclonal Antibody - Couverture souple

Dharshanan, Suba

 
9783659213113: Development and Production of Humanized Monoclonal Antibody: Development, Expression and Characterization of Humanized Anti-C2 Monoclonal Antibody
Couverture souple
ISBN 10 : 365921311X ISBN 13 : 9783659213113
Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

The treatment of cancer remains a formidable challenge owing to the difficulties in differentiating tumor from non-tumor cells in order to ameliorate the disease without causing intolerable toxicity to human patients. The use of monoclonal antibodies (mAbs) represents an attractive approach of overcoming this problem as the mAbs can be designed to selectively target tumor cells and elicit a variety of responses once bound. However, when rodent mAbs are administered in humans, anti-antibody responses are frequently observed. Hence, humanized monoclonal antibodies are instead widely used for diagnosis and treatment of cancer due to their reduced immunogenicity and high specificity. In this book, two humanization methods were utilized for the development of humanized mAb (hum-C2 mAbs) against C2-antigen, a marker expressed in colorectal and ovarian cancer, from mAb of mouse origin. Various procedures were also developed to produce hum-C2 mAbs, with high efficiency in terms continuity of production, lowered cost and increased speed. In addition, high-throughput and automated systems were also utilized to facilitate the upstream and downstream phases of hum-C2 mAbs.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

The treatment of cancer remains a formidable challenge owing to the difficulties in differentiating tumor from non-tumor cells in order to ameliorate the disease without causing intolerable toxicity to human patients. The use of monoclonal antibodies (mAbs) represents an attractive approach of overcoming this problem as the mAbs can be designed to selectively target tumor cells and elicit a variety of responses once bound. However, when rodent mAbs are administered in humans, anti-antibody responses are frequently observed. Hence, humanized monoclonal antibodies are instead widely used for diagnosis and treatment of cancer due to their reduced immunogenicity and high specificity. In this book, two humanization methods were utilized for the development of humanized mAb (hum-C2 mAbs) against C2-antigen, a marker expressed in colorectal and ovarian cancer, from mAb of mouse origin. Various procedures were also developed to produce hum-C2 mAbs, with high efficiency in terms continuity of production, lowered cost and increased speed. In addition, high-throughput and automated systems were also utilized to facilitate the upstream and downstream phases of hum-C2 mAbs.

Biographie de l'auteur

Dr. Dharshan is currently a Senior Research Scientist at Department of Protein Science, Inno Biologics. At the National University of Malaysia he earned a Bachelor of Science degree (Microbiology) in 2008 and was awarded Distinction. In 2012, he received his Ph.D. from the Faculty of Medicine, University of Malaya and was also awarded Distinction.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2012
Langue
anglais
ISBN 10 
365921311X
ISBN 13 
9783659213113
Reliure
Broché
Nombre de pages
348
Coordonnées du fabricant
non disponible
Personne responsable
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