Effect of Preservatives on Mitochondrial DNA: Comparative study on the effect of preservatives on mitochondrial DNA of spider Hippasa agelenoides (Lycosidae: Araneae) - Couverture souple

T. V., Vineeth Kumar; James, Remya; P. A., Sebastian

Couverture souple

ISBN 10 : 3659255106 ISBN 13 : 9783659255106

Editeur : LAP LAMBERT Academic Publishing, 2012

Synopsis

Earlier morphological features were the only criteria for taxonomical classification. Following the proposal of the structure, physical and chemical properties of DNA in the earlier half of twentieth century, that marked the beginning of molecular taxonomy. Morphological features are results of gene expression; molecular taxonomy can deliver a more precise and more accurate classification. For this the nuclear material should maintain its integrity. Hence it is necessary to preserve DNA with minimal degradation. In this scenario taxonomical studies demand a preservative that causes less harm to the DNA. Our study aims at comparing the effect of three routinely used preservatives - acetone, 70% ethanol and 4% formaldehyde. By comparing their effects we would identify which one among the three has a dual role as a good preservative and pose minimum damage to the DNA. Here we also compared the feasibility of four different extraction methods (DNeasy, Quick Extract, Master Pure and CTAB)

Détails de l'édition

• Éditeur: LAP LAMBERT Academic Publishing
• Date d'édition: 2012
• Langue: anglais
• ISBN 10: 3659255106
• ISBN 13: 9783659255106
• Reliure: Broché
• Nombre de pages: 64
• Coordonnées du fabricant: non disponible
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