Genetic Characterization Of Capsicum Germplasm Using Molecular Markers - Couverture souple

Akbar, Nazia; Ahmad, Habib; Ahmad, Imtiaz

 
9783659297380: Genetic Characterization Of Capsicum Germplasm Using Molecular Markers
Couverture souple
ISBN 10 : 3659297380 ISBN 13 : 9783659297380
Editeur : LAP LAMBERT Academic Publishing, 2014

Synopsis

Phylogenetic relationship among local and exotic genotypes of Capsicum were elaborated through morphological characterization and molecular markers.Protocols for isolation of total genomic DNA and seed storage proteins were optimized for Capsicum genotypes. Four RAPD primers were used to amplify genomic DNA. Unweighted Pair Group of Arithmetic Means (UPGMA) estimates of Genetic distances (GD) using RAPD primers ranged from 0 to 100%. Total seed storage proteins profile of the genotypes using SDS-PAGE analysis showed high range of genetic distances i.e. from 0–100% among various comparisons.The data obtained from PCR and SDS-PAGE was also used to study phylogenetic relationship through constructing two independent dendrograms. Dendrogram based on DNA data showed that the twenty-five genotypes were clustered into four groups.No such grouping was visible in the dendrogram based on the SDS-PAGE. Hence it was concluded that DNA based data is more useful for phylogenetic elaboration of Capsicum genotypes. Further morphogenetic and taxonomic study is recommended for assessment of the exact taxonomic status of genotypes segregated into different groups.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Phylogenetic relationship among local and exotic genotypes of Capsicum were elaborated through morphological characterization and molecular markers.Protocols for isolation of total genomic DNA and seed storage proteins were optimized for Capsicum genotypes. Four RAPD primers were used to amplify genomic DNA. Unweighted Pair Group of Arithmetic Means (UPGMA) estimates of Genetic distances (GD) using RAPD primers ranged from 0 to 100%. Total seed storage proteins profile of the genotypes using SDS-PAGE analysis showed high range of genetic distances i.e. from 0–100% among various comparisons.The data obtained from PCR and SDS-PAGE was also used to study phylogenetic relationship through constructing two independent dendrograms. Dendrogram based on DNA data showed that the twenty-five genotypes were clustered into four groups.No such grouping was visible in the dendrogram based on the SDS-PAGE. Hence it was concluded that DNA based data is more useful for phylogenetic elaboration of Capsicum genotypes. Further morphogenetic and taxonomic study is recommended for assessment of the exact taxonomic status of genotypes segregated into different groups.

Biographie de l'auteur

Nazia Akbar PhD Scholar, Department of Genetics Hazara University, Mansehra.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2014
Langue
anglais
ISBN 10 
3659297380
ISBN 13 
9783659297380
Reliure
Broché
Nombre de pages
112
Coordonnées du fabricant
non disponible
Personne responsable
non disponible