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Agrobacterium-Mediated Transformation Of Reporter Gene In Corn - Couverture souple

Ashraf, Faryam; Naz, Shagufta; Saleem, Faiza

 
9783659307669: Agrobacterium-Mediated Transformation Of Reporter Gene In Corn
Couverture souple
ISBN 10 : 3659307661 ISBN 13 : 9783659307669
Editeur : LAP LAMBERT Academic Publishing, 2014

Synopsis

Zea mays is the most economically important annual crop. A vector containing GUS reporter gene was introduce into corn via Agrobacterium mediated transformation.Transformants of different varieties of maize will be efficiently produced.The transformants will be histochemically detected for the presence of GUS reporter gene.Some conditions for best Agrobacterium mediated transformation was standardized using Agrobacterium tumefaciens strain EHA101 harboring binary vector Pzy102 containing GUS as reporter gene in kashmiri variety of corn. Numerous factors were compared and optimized for effecting the transfer of T-DNA and its incorporation to the genomic system of corn such as immature embryos of 2.5mm size were analyzed as explants of choice,100μM acetosyringone concentrations, N6 ad MS media composition, Clorox concentrations of 30%, co-cultivation durations of 3 days under dark at 22°C and bacterial concentrations was adjusted to get OD550 0.36, GUS chemical composition and incubation temperature of 37°C during GUS analysis were assessed as best. Different optimal experimental conditions were establish to enhance the β-glucuronidase (GUS) activity after transformation.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Zea mays is the most economically important annual crop. A vector containing GUS reporter gene was introduce into corn via Agrobacterium mediated transformation.Transformants of different varieties of maize will be efficiently produced.The transformants will be histochemically detected for the presence of GUS reporter gene.Some conditions for best Agrobacterium mediated transformation was standardized using Agrobacterium tumefaciens strain EHA101 harboring binary vector Pzy102 containing GUS as reporter gene in kashmiri variety of corn. Numerous factors were compared and optimized for effecting the transfer of T-DNA and its incorporation to the genomic system of corn such as immature embryos of 2.5mm size were analyzed as explants of choice,100μM acetosyringone concentrations, N6 ad MS media composition, Clorox concentrations of 30%, co-cultivation durations of 3 days under dark at 22°C and bacterial concentrations was adjusted to get OD550 0.36, GUS chemical composition and incubation temperature of 37°C during GUS analysis were assessed as best. Different optimal experimental conditions were establish to enhance the β-glucuronidase (GUS) activity after transformation.

Biographie de l'auteur

Miss Faryam Ashraf has done MS(Biotechnology) from Lahore College for Women University Lahore.She did her BS in zoology. Her particular area of research is to optimize the conditions for Agrobacterium-mediated transformation of reporter gene in corn.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2014
Langue
anglais
ISBN 10 
3659307661
ISBN 13 
9783659307669
Reliure
Broché
Nombre de pages
156
Coordonnées du fabricant
non disponible
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