Structural dynamics of intrinsically disordered proteins: The UmuD proteins are molecular adaptors in the regulation of DNA damage tolerance - Couverture souple

Ollivierre, Jaylene

Couverture souple

ISBN 10 : 3659326062 ISBN 13 : 9783659326066

Editeur : LAP Lambert Academic Publishing, 2013

Synopsis

The homodimeric umuD gene products play key roles in regulating the cellular response to DNA damage in Escherichia coli. UmuD2 is composed of 139-amino acid subunits and is upregulated as part of the SOS DNA damage response. Subsequently, damage-induced RecA:ssDNA nucleoprotein filaments mediate the slow autocleavage of the N-terminal 24-amino acid arms of UmuD2 yielding UmuD′2. It was previously proposed that UmuD cleaves only in the trans conformation, in which the arm of one monomer utilizes that active site of the adjacent monomer for cleavage. Cleavage in trans would therefore require dimerization. However, isoenergetic models of UmuD2 suggested that the arms may adopt cis (intramolecular) or trans (intermolecular) conformations, and may be unbound from or bound to the globular C-terminal domain. The dynamic nature of the N-terminal arms may explain how a number of distinct protein-protein contacts that prevent and facilitate mutagenic translesion synthesis (TLS) are made. Here we discuss how the conformation and dynamics of the UmuD proteins regulate the DNA damage response.

A propos du livre

À propos de l?auteur

Jaylene Ollivierre, PhD: Studied Biological Chemistry at Northeastern University. Postdoctoral researcher at MIT, Boston.

Détails de l'édition

• Éditeur: LAP Lambert Academic Publishing
• Date d'édition: 2013
• Langue: anglais
• ISBN 10: 3659326062
• ISBN 13: 9783659326066
• Reliure: Broché
• Nombre de pages: 168
• Coordonnées du fabricant: non disponible
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