Identification of candidate genes in stem cell proliferation and CR - Couverture souple

Plank, Michael

 
9783659337161: Identification of candidate genes in stem cell proliferation and CR
Couverture souple
ISBN 10 : 3659337161 ISBN 13 : 9783659337161
Editeur : LAP LAMBERT Academic Publishing, 2013

Synopsis

The process of aging is one of the least understood phenomena in biology. This work makes use of two important findings in the field of aging research: First of the conclusion that alterations in the proliferation of stem cells might be linked to the aging process, second that caloric restriction (CR) is a powerful intervention to extend life-span and delay aging associated diseases. In the first part we analysed a shRNA based screen to identify genes involved in the proliferation of stem cells and undertook a flow cytometry based proliferation assay to validate candidates. Secondly we meta-analysed microarray data on different experiments testing gene expression changes associated with CR. In general the obtained candidate genes and categories overlap with previous findings in the field such as the Ghr gene and categories related to lipid metabolism and insulin signaling and therefore suggest biological meaningfulness of the approach. On the other hand, novel functions like xenobiotic metabolism, circadian clock and retinol metabolism are promising to follow up on in the future. Some of the significant genes might play major roles as regulators of important signaling pathways.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

The process of aging is one of the least understood phenomena in biology. This work makes use of two important findings in the field of aging research: First of the conclusion that alterations in the proliferation of stem cells might be linked to the aging process, second that caloric restriction (CR) is a powerful intervention to extend life-span and delay aging associated diseases. In the first part we analysed a shRNA based screen to identify genes involved in the proliferation of stem cells and undertook a flow cytometry based proliferation assay to validate candidates. Secondly we meta-analysed microarray data on different experiments testing gene expression changes associated with CR. In general the obtained candidate genes and categories overlap with previous findings in the field such as the Ghr gene and categories related to lipid metabolism and insulin signaling and therefore suggest biological meaningfulness of the approach. On the other hand, novel functions like xenobiotic metabolism, circadian clock and retinol metabolism are promising to follow up on in the future. Some of the significant genes might play major roles as regulators of important signaling pathways.

Biographie de l'auteur

Michael Plank was born in Regensburg, Germany.He studied Biochemistry and Molecular Biology at the Universities of Regensburg and Vienna.His Diploma Thesis was in Dr de Magalhaes` group, University of Liverpool.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2013
Langue
anglais
ISBN 10 
3659337161
ISBN 13 
9783659337161
Reliure
Broché
Nombre de pages
120
Coordonnées du fabricant
non disponible
Personne responsable
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