Agrobacterium mediated transformation of Rice IR-64 and swarna - Couverture souple

Kumar, Kshitij; Singh, K.N.

 
9783659359088: Agrobacterium mediated transformation of Rice IR-64 and swarna
Couverture souple
ISBN 10 : 3659359084 ISBN 13 : 9783659359088
Editeur : LAP LAMBERT Academic Publishing, 2013

Synopsis

The present investigation was under taken to evolve an efficient method for Indica rice transformation. Agrobacterium-mediated methods used for rice transformation .Embryogenic calli were produced from mature seeds of IR64 and swarna (Indica varities) were used as a target cell in transformation experiments. MS medium supplemented with 2,4-D, (2mg/l) kinetin, (0.4mg/l). L. Proline, (500mg/L) Casein Hydrolysate, (300mg/L) agar-agar (0.8%w/v) was found best callus medium for swarna. MS medium supplemented with 2,4-D, (2mg/L) L-Proline (500mg/L) maltose (30g/L) agar–agar (0.8%w/v) was found to be best callus medium for IR64. Embryogenic calli were co cultivated with the agrobacterium strain LBA (4404) EHA105 using the protocol developed by Hiei et al, (1994) which was tested for applicability to IR64 and swarna Transformation.GUS assay was done with transformed calli and was better in swarna.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

The present investigation was under taken to evolve an efficient method for Indica rice transformation. Agrobacterium-mediated methods used for rice transformation .Embryogenic calli were produced from mature seeds of IR64 and swarna (Indica varities) were used as a target cell in transformation experiments. MS medium supplemented with 2,4-D, (2mg/l) kinetin, (0.4mg/l). L. Proline, (500mg/L) Casein Hydrolysate, (300mg/L) agar-agar (0.8%w/v) was found best callus medium for swarna. MS medium supplemented with 2,4-D, (2mg/L) L-Proline (500mg/L) maltose (30g/L) agar–agar (0.8%w/v) was found to be best callus medium for IR64. Embryogenic calli were co cultivated with the agrobacterium strain LBA (4404) EHA105 using the protocol developed by Hiei et al, (1994) which was tested for applicability to IR64 and swarna Transformation.GUS assay was done with transformed calli and was better in swarna.

Biographie de l'auteur

Kshitij Kumar is a Research Scholar at Department of Plant Molecular Biology Narendra Deva University of Agriculture & Technology Narendra Nagar (Kumarganj) Faizabad Uttarpradesh India. Mr. Kshitij Kumar has completed Msc (Agriculture)Biotechnology from NDUA&T and M.Phil Botany from University of Delhi.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2013
Langue
anglais
ISBN 10 
3659359084
ISBN 13 
9783659359088
Reliure
Broché
Nombre de pages
112
Coordonnées du fabricant
non disponible
Personne responsable
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