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RP-HPLC method for the determination of Anti-Anginal Drugs - Couverture souple

Amrohi, S.Hasan; Nazneen, Afra; Rasheed, Anas

 
9783659359651: RP-HPLC method for the determination of Anti-Anginal Drugs
Couverture souple
ISBN 10 : 3659359653 ISBN 13 : 9783659359651
Editeur : LAP LAMBERT Academic Publishing, 2013

Synopsis

A simple, specific, accurate and precise stability indicating reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous estimation of Aspirin and Isosorbide 5-mononitrate in bulk drug and its pharmaceutical dosage form. A chromatographic separation was achieved with reverse phase phenomenex® Luna 5µ C18 (2) 100A (250 × 4.60 mm) column in an isocratic mode at ambient temperature. The mobile phase consisting of water: methanol: acetonitrile (55:28:17% v/v/v) at a flow rate of 1 ml/min. The eluents were monitored at 217 nm. The retention times of Aspirin and Isosorbide 5-mononitrate were found to be 2.05±0.056 min and 4.27±0.016 min respectively. The regression analysis revealed linearity in the concentration range of 1-10 µg/ml and 1-10 µg/ml for Aspirin and Isosorbide 5-mononitrate respectively. The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) in accordance with ICH guide lines. The results of the study showed that the developed method is simple, precise and accurate, and therefore suitable for routine analysis of these drugs in pharmaceutical dosage form.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

A simple, specific, accurate and precise stability indicating reverse phase high performance liquid chromatographic (RP-HPLC) method has been developed for the simultaneous estimation of Aspirin and Isosorbide 5-mononitrate in bulk drug and its pharmaceutical dosage form. A chromatographic separation was achieved with reverse phase phenomenex® Luna 5µ C18 (2) 100A (250 × 4.60 mm) column in an isocratic mode at ambient temperature. The mobile phase consisting of water: methanol: acetonitrile (55:28:17% v/v/v) at a flow rate of 1 ml/min. The eluents were monitored at 217 nm. The retention times of Aspirin and Isosorbide 5-mononitrate were found to be 2.05±0.056 min and 4.27±0.016 min respectively. The regression analysis revealed linearity in the concentration range of 1-10 µg/ml and 1-10 µg/ml for Aspirin and Isosorbide 5-mononitrate respectively. The method was validated in terms of linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ) in accordance with ICH guide lines. The results of the study showed that the developed method is simple, precise and accurate, and therefore suitable for routine analysis of these drugs in pharmaceutical dosage form.

Biographie de l'auteur

S.Hasan Amrohi obtained his Masters in Pharmaceutical Analysis & Quality Assurance in Lalitha college of Pharmacy. He is currently working as a Assistant Professor at School of Pharmacy, Anurag Group Of Institution. Research interests are primarily in the areas of RP-HPLC, GC, LC-MS/MS, Drug Discovery, Stability Studies and Impurity Profiling.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2013
Langue
anglais
ISBN 10 
3659359653
ISBN 13 
9783659359651
Reliure
Broché
Nombre de pages
112
Coordonnées du fabricant
non disponible
Personne responsable
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