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Effect of Induced Mutation for Varietal Improvement of Grapes: Varietal Improvement by Mutagenesis - Couverture souple

Safdar, Ifrah; Munir, Neelma; Naz, Shagufta

 
9783659365997: Effect of Induced Mutation for Varietal Improvement of Grapes: Varietal Improvement by Mutagenesis
Couverture souple
ISBN 10 : 3659365998 ISBN 13 : 9783659365997
Editeur : LAP LAMBERT Academic Publishing, 2013

Synopsis

In vitro propagation of vitis is applied to increase plant material for cultivation. Cultures were maintained in vitro on MS medium supplemented with various growth regulators such as 2,4-D, BAP, NAA, TDZ and Kin. Comparatively all the hormones alone or in combinations were tried on grape varieties to examine their effects on initiation, as well as on multiplication. The 100% explant sterilization was achieved when free leaf explants of V1 and V2 exposed to 5% sodium hypochlorite for the duration of 5 minute. For V3 the same combination of treatment was found to be effective with 90 % survival of explants. It was observed during the present study that the nodal explants of all the three grape varieties gave 100% contamination free survival when they were treated with 5% sodium hypochlorite for 10 minutes. Best Shoot induction was observed in MS 1 medium i.e BAP 1mg/l. While maximum rooting was observed in NAA 1mg/l. The highest callus induction by leaf explants was 95% with V2 on 2.4-D 3mg/l followed by nodal segments on BA 1mg/l + NAA 0.5mg/l. Callogenesis from nodal explants regenerated in roots. By physical mutagenesis it was noted that doses up to 5 Gy helped to increase callus

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

In vitro propagation of vitis is applied to increase plant material for cultivation. Cultures were maintained in vitro on MS medium supplemented with various growth regulators such as 2,4-D, BAP, NAA, TDZ and Kin. Comparatively all the hormones alone or in combinations were tried on grape varieties to examine their effects on initiation, as well as on multiplication. The 100% explant sterilization was achieved when free leaf explants of V1 and V2 exposed to 5% sodium hypochlorite for the duration of 5 minute. For V3 the same combination of treatment was found to be effective with 90 % survival of explants. It was observed during the present study that the nodal explants of all the three grape varieties gave 100% contamination free survival when they were treated with 5% sodium hypochlorite for 10 minutes. Best Shoot induction was observed in MS 1 medium i.e BAP 1mg/l. While maximum rooting was observed in NAA 1mg/l. The highest callus induction by leaf explants was 95% with V2 on 2.4-D 3mg/l followed by nodal segments on BA 1mg/l + NAA 0.5mg/l. Callogenesis from nodal explants regenerated in roots. By physical mutagenesis it was noted that doses up to 5 Gy helped to increase callus

Biographie de l'auteur

Ifrah is a self-obsessed,motivated and confident student of M.S at L.C.W.U with target oriented traits.she excel in curricular & co curricular activities;have experience in dealing with scientific queries and awareness on recent scientific success and it's all possible by the toil of her renowned mentors Dr.Neelma Munir & Dr.Shagufta Naz

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2013
Langue
anglais
ISBN 10 
3659365998
ISBN 13 
9783659365997
Reliure
Broché
Nombre de pages
116
Coordonnées du fabricant
non disponible
Personne responsable
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