Novel mplex PCR-RFLP method for detect of MTHFR 677C>T,482G>A Variant - Couverture souple

Dubey, Widhi; Jain, Kapil

 
9783659376429: Novel mplex PCR-RFLP method for detect of MTHFR 677C>T,482G>A Variant
Couverture souple
ISBN 10 : 3659376426 ISBN 13 : 9783659376429
Editeur : LAP LAMBERT Academic Publishing, 2013

Synopsis

Methylene Tetra Hydro Folate Reductase (MTHFR) is an enzyme that in humans is encoded by the MTHFR gene. MTHFR catalyzes the conversion of 5, 10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular diseases, neural tube defects, colon cancer and acute leukemia, and mutations. This gene is associated with Methylenetetrahydrofolate reductase deficiency. Two kind of functional polymorphism, 677 C > T and 482 G > T have shown effect to severe disease in Human including cancers. Because of this enzyme deficiency cause many problems, till now 40 types of fatal disorders have been found in Human species. Main aim to do this project is to develop the new and novel method of detection of MTHFR gene related mutations at early stage. For this, 25 peripheral blood samples were randomly collected among the Indian population and DNA was isolated and the genotype polymorphism was determined by Multiplex-PCR RFLP method.

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Présentation de l'éditeur

Methylene Tetra Hydro Folate Reductase (MTHFR) is an enzyme that in humans is encoded by the MTHFR gene. MTHFR catalyzes the conversion of 5, 10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine. Genetic variation in this gene influences susceptibility to occlusive vascular diseases, neural tube defects, colon cancer and acute leukemia, and mutations. This gene is associated with Methylenetetrahydrofolate reductase deficiency. Two kind of functional polymorphism, 677 C > T and 482 G > T have shown effect to severe disease in Human including cancers. Because of this enzyme deficiency cause many problems, till now 40 types of fatal disorders have been found in Human species. Main aim to do this project is to develop the new and novel method of detection of MTHFR gene related mutations at early stage. For this, 25 peripheral blood samples were randomly collected among the Indian population and DNA was isolated and the genotype polymorphism was determined by Multiplex-PCR RFLP method.

Biographie de l'auteur

Prof(Dr)Widhi Dubey-Acad.Dir.@Mahatma Gandhi Inst.of Applied Sc.(JECRC-Found.)Jaipur, NAAC accredited “A” grade,Engaged in both PG & UG classes in Botany & Biotech.Published >16 research papers in Nat'l & Int'l Journals of repute.Author of books.Actively engaged in Research.Kapil Jain Co-author is meritorious career oriented PG-Biotech studentMGIaS

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2013
Langue
anglais
ISBN 10 
3659376426
ISBN 13 
9783659376429
Reliure
Broché
Nombre de pages
96
Coordonnées du fabricant
non disponible
Personne responsable
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