A New dot-ELISA Kit: The most sensitive & specific kit for the detection of antibodies against NDV, IBV and AIV, with several advantages - Couverture souple

Alam, Jehan; Hasan, Najmul

 
9783659403088: A New dot-ELISA Kit: The most sensitive & specific kit for the detection of antibodies against NDV, IBV and AIV, with several advantages
Couverture souple
ISBN 10 : 3659403083 ISBN 13 : 9783659403088
Editeur : LAP Lambert Academic Publishing, 2013

Synopsis

Poultry farming is the most dynamic & 2nd major industry in Pakistan. It has challenges of outbreaks of viral diseases resulting in high mortality, morbidity and huge economic loss in Pakistan. Conventional diagnostic tests are expensive, laborious & require technical expertise. Hence, a highly sensitive & specific dot-ELISA kit is developed, in two steps; first whole antigen is prepared from AIV, NDV and IBV and is applied onto nitrocellulose membrane; second rabbit anti-chicken antibodies is raised, purified and conjugated with enzyme HRP. Dot ELISA results are much comparative with ELISA & have advantages of high specificity, sensitivity & facile. This kit has the advantage of loading multiple antigens from local isolates & can be performed even at poultry forms; with no technical expertise or advance laboratory facilities for interpretations. Even the staff can test their bird’s immune response after vaccination or test for the presence of infection. It can be prepared & used on large scale; this combine approach of several antigens on single strip play a vital role in the diagnosis of poultry diseases or determining immune status and help in controlling diseases in Pakistan.

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À propos de l?auteur

Jehan Alam did M.Sc & MS degree from Microbiology department University of Karachi & worked as a research associate in Seoul National University & doing PhD studies in the field of molecular immunology and microbiology. Najmul Hasan is a seasoned microbiologist/chemist & author of several articles in both fields in world renowned journals.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP Lambert Academic Publishing
Date d'édition
2013
Langue
anglais
ISBN 10 
3659403083
ISBN 13 
9783659403088
Reliure
Broché
Nombre de pages
120
Coordonnées du fabricant
non disponible
Personne responsable
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