Characterization and molecular study of a Chemolithotrophic bacterium: Identification of a diheme Cytochrome c in a gram negative facultative chemolithotroph - Couverture souple

Banerjee, Rajarshi; Kothari, Indravadan L.; Mukhopadhyaya, Pratap N.

 
9783659415227: Characterization and molecular study of a Chemolithotrophic bacterium: Identification of a diheme Cytochrome c in a gram negative facultative chemolithotroph
Couverture souple
ISBN 10 : 3659415227 ISBN 13 : 9783659415227
Editeur : LAP LAMBERT Academic Publishing, 2013

Synopsis

A gram negative chemolithotrophic bacterium (RP1) with facultative mode of nutrition was isolated from the soil. Enzymological studies confirmed presence of Thiosulphate oxidase, sulphite oxidase and Rhodanese, all of which play role in sulphur metabolism pathway. A set of degenerate oligonucleotide primer pairs was used for thermal amplification of a major part of the coding region of the Cytochrome c gene locus of this bacterium. Nucleotide and translated amino acid sequence revealed the gene to be a diheme Cytochrome c, different from the monoheme Cytochrome c observed in Chloribium limicola, a photosynthetic green sulphur bacterium. Significant homology at the nucleotide level could be detected only with Pseudoaminobacter salicylatoxidans. On the contrary, significant homology at the amino acid level was observed with Bradyrhizobium japonicum, Silicobacter pomeroyi apart from P. salicylatoxidans. This is possibly because of codon degeneracy observed within the diverse members of chemolithotrophs. Greater homology at amino acid level with P. salicylatoxidans and B. japonicum compared to that with P.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

A gram negative chemolithotrophic bacterium (RP1) with facultative mode of nutrition was isolated from the soil. Enzymological studies confirmed presence of Thiosulphate oxidase, sulphite oxidase and Rhodanese, all of which play role in sulphur metabolism pathway. A set of degenerate oligonucleotide primer pairs was used for thermal amplification of a major part of the coding region of the Cytochrome c gene locus of this bacterium. Nucleotide and translated amino acid sequence revealed the gene to be a diheme Cytochrome c, different from the monoheme Cytochrome c observed in Chloribium limicola, a photosynthetic green sulphur bacterium. Significant homology at the nucleotide level could be detected only with Pseudoaminobacter salicylatoxidans. On the contrary, significant homology at the amino acid level was observed with Bradyrhizobium japonicum, Silicobacter pomeroyi apart from P. salicylatoxidans. This is possibly because of codon degeneracy observed within the diverse members of chemolithotrophs. Greater homology at amino acid level with P. salicylatoxidans and B. japonicum compared to that with P.

Biographie de l'auteur

Dr. Rajarshi Banerjee was awarded his PhD degree in Microbiology from Sardar Patel University, Gujarat, India in the year 2006.Dr. IL Kothari is an eminent scientist in the area of biotechnology and has over 50 research articles to his credit. Dr. Pratap N Mukhopadhyaya hold a PhD degree in Science from Jadavpur University, India.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2013
Langue
anglais
ISBN 10 
3659415227
ISBN 13 
9783659415227
Reliure
Broché
Nombre de pages
104
Coordonnées du fabricant
non disponible
Personne responsable
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