Transcriptomic analysis of defense mechanism in sugarcane - Couverture souple

Rahul, P.R.; Viswanathan, Rasappa

 
9783659415791: Transcriptomic analysis of defense mechanism in sugarcane
Couverture souple
ISBN 10 : 3659415790 ISBN 13 : 9783659415791
Editeur : LAP LAMBERT Academic Publishing, 2013

Synopsis

A new method of in vitro interaction between cell suspension and Cf-elicitor was employed to understand red rot resistance in sugarcane. RT-PCR, differential display (DD)-RT-PCR, RACE-PCR, semi-quantitative RT-PCR and different bioinformatic tools were used to identify and characterize the defense related transcripts. In DD-RT-PCR, many differentially expressed transcripts were identified and functionally grouped. Important candidate genes were identified through RACE-PCR and their functional domains were characterized. Sugarcane chitinase was characterized as a class IV chitinase. Differential accumulation of several transcripts of the flavanoid biosynthetic pathway was established through spatial expression by semi quantitative RT-PCR and this study further confirms the role of sugarcane phytoalexins in red rot resistance. The study has resulted in the generation of large number of TDFs that can be used in future studies in sugarcane-C. falcatum interaction as well as well as other biotic/abiotic stress factors. For the first time, this study established that in vitro interaction between cell suspension and Cf-elicitor mimics molecular interaction in cane tissue and C. falcatum.

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Présentation de l'éditeur

A new method of in vitro interaction between cell suspension and Cf-elicitor was employed to understand red rot resistance in sugarcane. RT-PCR, differential display (DD)-RT-PCR, RACE-PCR, semi-quantitative RT-PCR and different bioinformatic tools were used to identify and characterize the defense related transcripts. In DD-RT-PCR, many differentially expressed transcripts were identified and functionally grouped. Important candidate genes were identified through RACE-PCR and their functional domains were characterized. Sugarcane chitinase was characterized as a class IV chitinase. Differential accumulation of several transcripts of the flavanoid biosynthetic pathway was established through spatial expression by semi quantitative RT-PCR and this study further confirms the role of sugarcane phytoalexins in red rot resistance. The study has resulted in the generation of large number of TDFs that can be used in future studies in sugarcane-C. falcatum interaction as well as well as other biotic/abiotic stress factors. For the first time, this study established that in vitro interaction between cell suspension and Cf-elicitor mimics molecular interaction in cane tissue and C. falcatum.

Biographie de l'auteur

Sugarcane Breeding Institute, Coimbatore

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2013
Langue
anglais
ISBN 10 
3659415790
ISBN 13 
9783659415791
Reliure
Broché
Nombre de pages
188
Coordonnées du fabricant
non disponible
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