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Detection and Molecular Characterisation of Vibrios: State of Molecular Methods in Vibrio Differentiation - Couverture souple

Paydar, Mohammadjavad

 
9783659497742: Detection and Molecular Characterisation of Vibrios: State of Molecular Methods in Vibrio Differentiation
Couverture souple
ISBN 10 : 3659497746 ISBN 13 : 9783659497742
Editeur : LAP LAMBERT Academic Publishing, 2014

Synopsis

Pathogenic Vibrio species are significant causes of gastroenteritis associated with consumption of under-cooked seafood especially in Southeast Asian countries, where the temperature is optimal for their growth. The objectives of this study were to investigate the prevalence of Vibrio spp. in raw seafood using conventional and molecular methods; to compare these methods based on their sensitivity and discriminatory power; and to analyse pathogenicity and genetic variability of V. parahaemolyticus isolates. We found that Vibrio spp. were identified more efficiently by CHROMagarTM Vibrio rather than thiosulfate-citrate-bile salts-sucrose (TCBS). API 20E indicated higher discriminatory power to differentiate V. cholerae, V. parahaemolyticus and V. vulnificus, compared to conventional biochemical tests. The gyrB/pntA genes-based multiplex PCR was confirmed as an accurate and efficient screening tool for detection of Vibrio spp. Virulent V. parahaemolyticus isolates were isolated from seafood demonstrating a potential risk to consumers. REP-PCR demonstrated high genetic diversity of the V. parahaemolyticus isolates and it was able to distinguish the isolates with different virulotypes.

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Présentation de l'éditeur

Pathogenic Vibrio species are significant causes of gastroenteritis associated with consumption of under-cooked seafood especially in Southeast Asian countries, where the temperature is optimal for their growth. The objectives of this study were to investigate the prevalence of Vibrio spp. in raw seafood using conventional and molecular methods; to compare these methods based on their sensitivity and discriminatory power; and to analyse pathogenicity and genetic variability of V. parahaemolyticus isolates. We found that Vibrio spp. were identified more efficiently by CHROMagarTM Vibrio rather than thiosulfate-citrate-bile salts-sucrose (TCBS). API 20E indicated higher discriminatory power to differentiate V. cholerae, V. parahaemolyticus and V. vulnificus, compared to conventional biochemical tests. The gyrB/pntA genes-based multiplex PCR was confirmed as an accurate and efficient screening tool for detection of Vibrio spp. Virulent V. parahaemolyticus isolates were isolated from seafood demonstrating a potential risk to consumers. REP-PCR demonstrated high genetic diversity of the V. parahaemolyticus isolates and it was able to distinguish the isolates with different virulotypes.

Biographie de l'auteur

Mohammadjavad Paydar holds a Master in Medical Biotechnology from University of Malaya (UM). His research interests particularly focus on Molecular Microbiology, Cellular Biology and Biomolecular Markers associated with Cancer Prognosis and Treatment.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2014
Langue
anglais
ISBN 10 
3659497746
ISBN 13 
9783659497742
Reliure
Broché
Nombre de pages
108
Coordonnées du fabricant
non disponible
Personne responsable
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