Process optimization for amylase production by Plackett-Burman design - Couverture souple

Fatima, Bilqees

 
9783659611889: Process optimization for amylase production by Plackett-Burman design
Couverture souple
ISBN 10 : 3659611883 ISBN 13 : 9783659611889
Editeur : LAP LAMBERT Academic Publishing, 2014

Synopsis

Sixteen different mould cultures viz. Aspergillus, Alternaria, Arthroderma, Trichoderma, Fusarium, Penicillium, Rhizopus and Chochliobolus were isolated from the soil samples collected from different localities of Doha (Qatar) by serial dilution method. The preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar medium by pour plate method. The isolates were then subjected to secondary screening by submerged fermentation. The product yield was analyzed in dependence of mycelial morphology, biomass level and protein contents. On the basis of kinetic variables, notably A. oryzae IIB-6 was found to be a hyper producer of GGH compared to A. kawachii IIB-2. A noticeable enhancement in enzyme activity of over 30% was observed when process parameters were further optimized using a 2-factorial Plackett-Burman design. The model terms were found to be highly significant (HS, p≤0.05), indicating the potential utility of the culture (dof~3).

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

Sixteen different mould cultures viz. Aspergillus, Alternaria, Arthroderma, Trichoderma, Fusarium, Penicillium, Rhizopus and Chochliobolus were isolated from the soil samples collected from different localities of Doha (Qatar) by serial dilution method. The preliminary screening of isolates was done by selecting initial colonies showing relatively bigger zones of starch hydrolysis on nutrient agar medium by pour plate method. The isolates were then subjected to secondary screening by submerged fermentation. The product yield was analyzed in dependence of mycelial morphology, biomass level and protein contents. On the basis of kinetic variables, notably A. oryzae IIB-6 was found to be a hyper producer of GGH compared to A. kawachii IIB-2. A noticeable enhancement in enzyme activity of over 30% was observed when process parameters were further optimized using a 2-factorial Plackett-Burman design. The model terms were found to be highly significant (HS, p≤0.05), indicating the potential utility of the culture (dof~3).

Biographie de l'auteur

Bilqees Fatima has a Bachelor of Science in Medical and Biological Sciences. A Master of Botany with a specialization in Fermentation Technology. A Master of Philosophy in Biotechnology/Biochemistry with a specialization in Protein Purification and a Ph.D in Biotechnology/Molecular Biology with a specialization in Genetic Engineering.PAKISTAN.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2014
Langue
anglais
ISBN 10 
3659611883
ISBN 13 
9783659611889
Reliure
Broché
Nombre de pages
88
Coordonnées du fabricant
non disponible
Personne responsable
KreGa Onlinehandel GmbH
4950000000000
info@krega-onlinehandel.de

Birkenweg 31
Brakel
33034
Allemagne