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Bioethanol production potential of termites bacteria utilizing corncob: bioethanol production potential using cellulosic waste - Couverture souple

Amjad, Maroof; Iqtedar, Mehwish; Naz, Shagufta

 
9783659621673: Bioethanol production potential of termites bacteria utilizing corncob: bioethanol production potential using cellulosic waste
Couverture souple
ISBN 10 : 3659621676 ISBN 13 : 9783659621673
Editeur : LAP LAMBERT Academic Publishing, 2014

Synopsis

The current study aimed at the lab scale production of bioethanol from corncob employing cellulolytic bacteria isolated from termite gut. Cellulolytic bacteria were isolated from two local termite species including Coptotermes heimi and Microtermes mycophagous. Corn cob was de-lignified. Two fold decrease in lignin and 20% increase in cellulose was obtained after pretreatment. Among six bacterial isolated CH-1 and MYC-1 were selected for their potential cellulolytic action through halo zone formation in primary screening and substrate hydrolysis during secondary screening. Temperature, pH, substrate concentration, inoculum size, surfactants, metal ions, PEG concentration, CMCase and FPase activity were optimized for CH-1 and MYC-1. Optimal bioethanol yield was 5.45 mg/ml and 5.79 mg/ml from CH-1 and MYC-1 respectively by simultaneous saccharification and fermentation. 16S rDNA sequence analysis has shown homology with Bacillus cereus (CH-1) and Providencia vermicola. (MYC-1). Therefore it can be concluded that both isolates had shown potential bioconversion of agro-waste into fermentable sugars that later could be fermented to bioethanol.

Les informations fournies dans la section « Synopsis » peuvent faire référence à une autre édition de ce titre.

Présentation de l'éditeur

The current study aimed at the lab scale production of bioethanol from corncob employing cellulolytic bacteria isolated from termite gut. Cellulolytic bacteria were isolated from two local termite species including Coptotermes heimi and Microtermes mycophagous. Corn cob was de-lignified. Two fold decrease in lignin and 20% increase in cellulose was obtained after pretreatment. Among six bacterial isolated CH-1 and MYC-1 were selected for their potential cellulolytic action through halo zone formation in primary screening and substrate hydrolysis during secondary screening. Temperature, pH, substrate concentration, inoculum size, surfactants, metal ions, PEG concentration, CMCase and FPase activity were optimized for CH-1 and MYC-1. Optimal bioethanol yield was 5.45 mg/ml and 5.79 mg/ml from CH-1 and MYC-1 respectively by simultaneous saccharification and fermentation. 16S rDNA sequence analysis has shown homology with Bacillus cereus (CH-1) and Providencia vermicola. (MYC-1). Therefore it can be concluded that both isolates had shown potential bioconversion of agro-waste into fermentable sugars that later could be fermented to bioethanol.

Biographie de l'auteur

Maroof Amjad was born in Lahore. She has done her MS in biotechnology from Lahore College for Women University Lahore, a well reputed university in Lahore with zeal and energy for science and research. Bio-sciences always fascinated her throughout her academic life. Her area of specialization in MS was industrial biotechnology.

Les informations fournies dans la section « A propos du livre » peuvent faire référence à une autre édition de ce titre.

Éditeur
LAP LAMBERT Academic Publishing
Date d'édition
2014
Langue
anglais
ISBN 10 
3659621676
ISBN 13 
9783659621673
Reliure
Broché
Nombre de pages
160
Coordonnées du fabricant
non disponible
Personne responsable
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